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Ubfd1 (NM_138589) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG205672
Ubfd1 (tGFP-tagged) - Mouse ubiquitin family domain containing 1 (Ubfd1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ubfd1
Synonyms AI467302; D7Wsu105e; D7Wsu128e; Ubph
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205672 representing NM_138589
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTAAAGCGTGGCAGGGGCCGGCCCGGGAAGCGGAGGAGGCGGGTCTCCATAGAAACCTCCACCTGTT
TCCGGCCAGCCTGTGTGAAATTAGGGGCTGGTGCGGGGGCCAATCTCCGCCAGCTCGCTTCTTCCCGGCG
GCCCCTGCGGTCGTGGTGGGTGTTGTACACAATCATCATGGCGGCGGCCGGAGCCCCGGATGGCATGGAG
GAGCCAGGCATGGACACGGAGGCCGAGGCTGTGGCCACCGAGGCGCCCGCACGGCCCCTCAACTGCGTGG
AGGCCGAAGCAGCCGTAGGGGCAGCGGCGGAGGACTCCTGCGACGCGCGAGGCAACCTGCAGCCGGCCCC
GGCCCAGCCCCCTGGGGACCCTGCGGCCCAGGCCTCGGTCAGCAACGGCGAGGACGCGGGCGGCGGTGTG
GGCAAGGAGCTGGTGGATCTGAAGATAATCTGGAACAAGACCAAACACGACGTGAAGGTCCCTCTGGACA
GCACGGGGTCCGAGCTAAAGCAGAAGATTCACTCCATTACAGGTCTCCCACCTGCCATGCAGAAGGTCAT
GTATAAAGGACTTGTTCCTGAAGACAAGACTTTGAGAGAAATAAAAGTGACCAGTGGGGCCAAGATCATG
GTGGTTGGTTCTACTATAAATGATGTTTTAGCAGTAAACACCCCGAAAGATGCTGCCCAGCAGGATGCAA
AGGCTGAAGAAAACAAGAAGGAGCCCCTCTGCAGGCAGAAGCAACACAGGAAAGTGTTGGACAAGGGAAA
GCCTGAAGATGTGATGCCGTCTGTTAAGGGTGCCCAGGAGCGCCTGCCAACGGTACCCTTGTCTGGCATG
TACAATAAGTCTGGCGGGAAAGTGAGGCTCACCTTTAAGCTAGAGCAAGACCAGCTGTGGATCGGCACTA
AAGAGCGGACTGAGAAACTGCCCATGGGCTCCATTAAAAATGTGGTCAGTGAACCTATTGAAGGACATGA
AGACTACCACATGATGGCGTTTCAGTTGGGCCCCACGGAAGCCTCTTACTACTGGGTGTACTGGGTTCCA
ACTCAATATGTGGATGCAATCAAAGACACTGTGCTGGGCAAGTGGCAGTATTTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205672 representing NM_138589
Red=Cloning site Green=Tags(s)

MLKRGRGRPGKRRRRVSIETSTCFRPACVKLGAGAGANLRQLASSRRPLRSWWVLYTIIMAAAGAPDGME
EPGMDTEAEAVATEAPARPLNCVEAEAAVGAAAEDSCDARGNLQPAPAQPPGDPAAQASVSNGEDAGGGV
GKELVDLKIIWNKTKHDVKVPLDSTGSELKQKIHSITGLPPAMQKVMYKGLVPEDKTLREIKVTSGAKIM
VVGSTINDVLAVNTPKDAAQQDAKAEENKKEPLCRQKQHRKVLDKGKPEDVMPSVKGAQERLPTVPLSGM
YNKSGGKVRLTFKLEQDQLWIGTKERTEKLPMGSIKNVVSEPIEGHEDYHMMAFQLGPTEASYYWVYWVP
TQYVDAIKDTVLGKWQYF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138589
ORF Size 1104 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138589.2, NP_613055.2
RefSeq Size 4831 bp
RefSeq ORF 1107 bp
Locus ID 28018
UniProt ID Q78JW9
Cytogenetics 7 65.35 cM
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"NM_138589" in other vectors (4)
SKU Description Size Price
MC204261 Ubfd1 (untagged) - Mouse ubiquitin family domain containing 1 (Ubfd1), (10ug) 10 ug
$457.00
MR205672 Ubfd1 (Myc-DDK-tagged) - Mouse ubiquitin family domain containing 1 (Ubfd1) 10 ug
$289.00 $457.00
MR205672L3 Lenti ORF clone of Ubfd1 (Myc-DDK-tagged) - Mouse ubiquitin family domain containing 1 (Ubfd1) 10 ug
$757.00
MR205672L4 Lenti ORF clone of Ubfd1 (mGFP-tagged) - Mouse ubiquitin family domain containing 1 (Ubfd1) 10 ug
$757.00

Plasmid Map for MG205672

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG205672
Circular map for MG205672

file-alt Citations

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