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Dus1l (BC019480) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG205618
Dus1l (tGFP-tagged) - Mouse dihydrouridine synthase 1-like (S. cerevisiae) (cDNA clone MGC:28421 IMAGE:4037583)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dus1l
Synonyms 1110032N12Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205618 representing BC019480
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATAGCCAAGAGAGGTCACTATGGTGCTTTTCTGCAGGAGGAGTGGGACCTTCTTCAAAGAATGATTC
TGTTGGCTCATGAGCGACTCTCTGTTCCTGTCACGTGCAAAATTCGTGTCTTCCCAGAAATTGACAAGAC
AGTGAGGTACGCCCAGATGCTGGAGAAGGCTGGCTGTCAGCTGCTGACTGTGCATGGGCGCACCAAGGAG
CAGAAGGGGCCCATGGCAGGAACAGCCTCCTGGGAACACATCAAGGCCGTTCGGAAGGCTGTGGGAATCC
CTGTGTTTGCCAATGGGAACATCCAATGCCTGCAGGATGTGGAGCGGTGCATCCAGGACACCGGCGTGCA
GGGAGTCATGAGTGCAGAGGGGAACCTGCACAACCCTGCCCTCTTTGAGGGCCGGAGTCCTGCTGTGTGG
GAGCTGGCCGAGGAGTACCTGGACATTGTGCGGCAACACCCTTGTCCACTATCGTATGTCCGGGCCCATC
TCTTCAAACTGTGGCACCACACGCTACAGGTACATCAGCAACTTCGAGAAGAGCTGGCCAAAGTGAAGAC
CCTGGAGGGCGTGGCTGCTGTGAGCCAGGCGCTAAAGCTTCGGTGTCAGGAGGACATGTCTAGGCAGCAA
GAAGGAGTGAGGCCAGCTGACAACTTACCTGCTTTCCATTGGATCTGCCAGCCGTACATCCGGCCAGGAC
CTAGGGAAGGGAGCAAGGAGAATAGTGGTGGTCGAAGCAAGCGGGCTCTGGAGGAAGAAGAAGGCAGCAT
GGAGGGCTTGTCCAAGAATAAGCTGAAGAAACAACTGAGGAACCCTCACAAAACCTTTGACCCTTCCCTG
AAACCCAAATATGCCAAGTGTGACCAGTGTGGAAATCCAAAGGGCAATAGGTGTGTGTTTAATCTGTGCC
GTGGCTGCTGCAAGAAGCGAGCTTTCAGAGAGACAGCAGATTGCCCAGGTCATGGATTGCTTTTTAAGAC
CAAATTAGAGAAGTCTTTGGCCTGGAAAGGGACCCAGCCTGGACTGCAGGAAGCCCAGCAGGTGAGGCCT
GTGACACCAAGTGGTTTCTCTGAAGTCGTGGGTAGTGCCCTAGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205618 representing BC019480
Red=Cloning site Green=Tags(s)

MIAKRGHYGAFLQEEWDLLQRMILLAHERLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKE
QKGPMAGTASWEHIKAVRKAVGIPVFANGNIQCLQDVERCIQDTGVQGVMSAEGNLHNPALFEGRSPAVW
ELAEEYLDIVRQHPCPLSYVRAHLFKLWHHTLQVHQQLREELAKVKTLEGVAAVSQALKLRCQEDMSRQQ
EGVRPADNLPAFHWICQPYIRPGPREGSKENSGGRSKRALEEEEGSMEGLSKNKLKKQLRNPHKTFDPSL
KPKYAKCDQCGNPKGNRCVFNLCRGCCKKRAFRETADCPGHGLLFKTKLEKSLAWKGTQPGLQEAQQVRP
VTPSGFSEVVGSALA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC019480
ORF Size 1095 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC019480, AAH19480
RefSeq Size 1423 bp
RefSeq ORF 1097 bp
Locus ID 68730
Cytogenetics 11 E2
Summary Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.UniProtKB/Swiss-Prot Function
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"BC019480" in other vectors (4)
SKU Description Size Price
MC217857 Dus1l (untagged) - Mouse dihydrouridine synthase 1-like (S. cerevisiae) (cDNA clone MGC:28421 IMAGE:4037583), (10ug) 10 ug
$503.00
MR205618 Dus1l (Myc-DDK-tagged) - Mouse dihydrouridine synthase 1-like (S. cerevisiae) (cDNA clone MGC:28421 IMAGE:4037583) 10 ug
$289.00 $457.00
MR205618L3 Lenti ORF clone of Dus1l (Myc-DDK-tagged) - Mouse dihydrouridine synthase 1-like (S. cerevisiae) (cDNA clone MGC:28421 IMAGE:4037583) 10 ug
$757.00
MR205618L4 Lenti ORF clone of Dus1l (mGFP-tagged) - Mouse dihydrouridine synthase 1-like (S. cerevisiae) (cDNA clone MGC:28421 IMAGE:4037583) 10 ug
$757.00

Plasmid Map for MG205618

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG205618
Circular map for MG205618

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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