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Rfc3 (NM_027009) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG205432
Rfc3 (tGFP-tagged) - Mouse replication factor C (activator 1) 3 (Rfc3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rfc3
Synonyms 38kDa; 2810416I22Rik; AU022547; Recc3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205432 representing NM_027009
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCTCTGGGTGGACAAGTACCGGCCGAGCTCCCTTGCCCGGCTGGACTATCATAAGGAACAGGCAG
CACAGCTGCGCAACCTGGTGCAGTGTGGAGACTTTCCTCACCTCTTAGTATATGGACCATCAGGTGCTGG
AAAAAAGACAAGAATTATGTGTATTCTACGTGAACTTTATGGTATCGGGGTGGAAAAACTGAGAATTGAG
CATCAGACTATCACAACCCCGTCTAAAAAAAAAATTGAAATCAGCACCATTGCAAGCAACTACCACCTTG
AAGTTAATCCCAGTGATGCGGGGAACAGCGACCGAGTTGTAATTCAGGAGATGCTGAAGACAGTGGCACA
GTCACAACAACTTGAAACCAGCTCACAAAGAGACTTCAAAGTGGTATTATTGACAGAGGTGGACAAACTC
ACAAAGGATGCTCAGCATGCCTTACGCAGAACTATGGAGAAATACATGTCTACCTGCAGGCTCATCTTGT
GCTGCAATTCCACATCTAAGGTGATACCCCCGATCCGAAGCAGGTGCCTGGCAGTTCGTGTGCCTGCTCC
CAGCATCGAAGATATTTGCAGTGTACTATCCACTGTCTGCAGGAAGGAAGGTCTGGCTCTCCCTTCGACA
TTGGCCCGTAGGCTGGCCGAGAAGTCATGCCGAAACCTCAGGAAAGCCTTACTTATGTGTGAGGCCTGCA
GAGTGCAGCAATACCCTTTTACGGAAGACCAAGAAATCCCAGAGACAGACTGGGAGGTGTATCTGAGGGA
GACCGCAAATGCTATTGTCAGTCAGCAGACGCCACAGAGGCTCCTTGAAGTCCGTGGAAGGCTCTATGAG
CTTCTAACTCATTGTATTCCTCCTGAGATAATAATGAAGGGCCTCCTCTCAGAACTCCTCCATAACTGTG
ATGGGCAGCTGAAAGGGGAAGTGGCACAGATGGCAGCCTACTATGAGCACCGACTACAGCTGGGCAGCAA
AGCCATTTATCATTTGGAAGCATTTGTGGCGAAGTTTATGGCACTTTATAAGAAGTTCATGGAGGATGGA
CTGGAAGGCATGATGTTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205432 representing NM_027009
Red=Cloning site Green=Tags(s)

MSLWVDKYRPSSLARLDYHKEQAAQLRNLVQCGDFPHLLVYGPSGAGKKTRIMCILRELYGIGVEKLRIE
HQTITTPSKKKIEISTIASNYHLEVNPSDAGNSDRVVIQEMLKTVAQSQQLETSSQRDFKVVLLTEVDKL
TKDAQHALRRTMEKYMSTCRLILCCNSTSKVIPPIRSRCLAVRVPAPSIEDICSVLSTVCRKEGLALPST
LARRLAEKSCRNLRKALLMCEACRVQQYPFTEDQEIPETDWEVYLRETANAIVSQQTPQRLLEVRGRLYE
LLTHCIPPEIIMKGLLSELLHNCDGQLKGEVAQMAAYYEHRLQLGSKAIYHLEAFVAKFMALYKKFMEDG
LEGMMF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027009
ORF Size 1068 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027009.1, NP_081285.1
RefSeq Size 1217 bp
RefSeq ORF 1071 bp
Locus ID 69263
UniProt ID Q8R323
Cytogenetics 5 G3
Summary The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.UniProtKB/Swiss-Prot Function
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"NM_027009" in other vectors (4)
SKU Description Size Price
MC202845 Rfc3 (untagged) - Mouse replication factor C (activator 1) 3 (Rfc3), (10ug) 10 ug
$457.00
MR205432 Rfc3 (Myc-DDK-tagged) - Mouse replication factor C (activator 1) 3 (Rfc3) 10 ug
$289.00 $457.00
MR205432L3 Lenti ORF clone of Rfc3 (Myc-DDK-tagged) - Mouse replication factor C (activator 1) 3 (Rfc3) 10 ug
$757.00
MR205432L4 Lenti ORF clone of Rfc3 (mGFP-tagged) - Mouse replication factor C (activator 1) 3 (Rfc3) 10 ug
$757.00

Plasmid Map for MG205432

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG205432
Circular map for MG205432

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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