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Sclt1 (BC043136) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG205320
Sclt1 (tGFP-tagged) - Mouse sodium channel and clathrin linker 1 (cDNA clone MGC:58077 IMAGE:6812105)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sclt1
Synonyms 2610207F23Rik; 4931421F20Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205320 representing BC043136
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACAGAAATCGACTTACTGAGAGACCAAAAAGATAAACTAAATGATATTCTTAGGCAGCATCAAA
TCGAACATATTTTCAGAGACCCAACTATGCAGAACAGCATGTCCAAAGGAGGCAGAGGTGACACACTAGT
AGACTCAATAAATGAGCAAAGTTCTTTACCTCCTCTCATTGCTGAGTATGAGAAACATCTGGAAGAGCTA
AACAGGCAGCTGACCTATTATCAGAAGCACATGGGTGAGATGAAACTACAACTTGAAACTGTCATCACAG
AAAATGAAAGGTTGCACAGTAAATTAAAAGATGCTGTTGAAAAGCAATTGGAAGCCCTTCCCTTTGGCAC
AGGGATAGGAAATGACATCTGTGCAGATGATGAGACCGTGAGGATCCTTCAAGAACAATTGCAACTAGCC
AATCAAGAGAAAACTTGGGCTCTGGAACTCTGGCAGACTGCTTCTCAGGAGTTGCAAAGTGTACAGAAAC
TCTATCAGGAGCATATGACTGAAGCCCAGATTCATGAATTTGAAAACCGAAAGCAAAAAGATCAGCTGAA
CAATTTTCAACAACTAACCAAGAAACTCCATGTTGCTAATGAGAACATAGAAATGACTAACCACCACTTC
CTGAAAACAGTAACTGAACAAAACATGGAGATTGAGAAACTTCGAAAACATCTTAGGCAAGCCAGATTAG
ATCTGAGAGTTGCAGTATCGAAAGTGGAAGAGTTAACTAAAGTGACTGAAGGTCTTCAAGAGCAGATGCT
GAAGAAGGAGGAAGATATTATGTCTGCACAAGGAAAAGAAGAAGCGTCGGATAGACGAGTACAGCAGTTA
CAATCTAGTATAAAACAGTTAGAGTCAAGATTGTGTGTAGCAATTGAAGAAGCCGATGTACTAAAAACAG
GGAAATCAAATTTGGAAAAGCAAATCAAAGAACTACAAGCAAAATGTAGTGAATCAGAAAATGAGAAATA
TGAGGCTATTTCAAGAGCCAGAGATAGCATGCAGCTCTTAGAAGAAGCTAACATTAAACAAAATCAGCTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205320 representing BC043136
Red=Cloning site Green=Tags(s)

MATEIDLLRDQKDKLNDILRQHQIEHIFRDPTMQNSMSKGGRGDTLVDSINEQSSLPPLIAEYEKHLEEL
NRQLTYYQKHMGEMKLQLETVITENERLHSKLKDAVEKQLEALPFGTGIGNDICADDETVRILQEQLQLA
NQEKTWALELWQTASQELQSVQKLYQEHMTEAQIHEFENRKQKDQLNNFQQLTKKLHVANENIEMTNHHF
LKTVTEQNMEIEKLRKHLRQARLDLRVAVSKVEELTKVTEGLQEQMLKKEEDIMSAQGKEEASDRRVQQL
QSSIKQLESRLCVAIEEADVLKTGKSNLEKQIKELQAKCSESENEKYEAISRARDSMQLLEEANIKQNQL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC043136
ORF Size 1050 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC043136, AAH43136
RefSeq Size 2783 bp
RefSeq ORF 1052 bp
Locus ID 67161
Cytogenetics 3 B
Summary Adapter protein that links SCN10A to clathrin. Regulates SCN10A channel activity, possibly by promoting channel internalization (By similarity).UniProtKB/Swiss-Prot Function
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"BC043136" in other vectors (4)
SKU Description Size Price
MC218375 Sclt1 (untagged) - Mouse sodium channel and clathrin linker 1 (cDNA clone MGC:58077 IMAGE:6812105), (10ug) 10 ug
$503.00
MR205320 Sclt1 (Myc-DDK-tagged) - Mouse sodium channel and clathrin linker 1 (cDNA clone MGC:58077 IMAGE:6812105) 10 ug
$289.00 $457.00
MR205320L3 Lenti ORF clone of Sclt1 (Myc-DDK-tagged) - Mouse sodium channel and clathrin linker 1 (cDNA clone MGC:58077 IMAGE:6812105) 10 ug
$757.00
MR205320L4 Lenti ORF clone of Sclt1 (mGFP-tagged) - Mouse sodium channel and clathrin linker 1 (cDNA clone MGC:58077 IMAGE:6812105) 10 ug
$757.00

Plasmid Map for MG205320

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG205320
Circular map for MG205320

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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