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Elavl2 (BC046598) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG205233
Elavl2 (tGFP-tagged) - Mouse ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B) (cDNA clone MGC:54659
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Elavl2
Synonyms Hub; mel-N1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205233 representing BC046598
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAACACAACTGTCTAATGGGCCAACTTGCAATAACACAGCCAATGGTCCAACCACTGTAAACAACA
ACTGCTCCTCACCAGTTGACTCCGGGAACACAGAGGACAGCAAGACCAACCTAATAGTCAACTACCTTCC
TCAGAACATGACACAGGAAGAACTAAAGAGTCTTTTTGGGAGCATTGGCGAGATAGAGTCCTGTAAGCTT
GTAAGAGACAAAATAACAGGGCAGAGCTTGGGTTACGGATTTGTGAACTACATTGACCCCAAGGATGCAG
AGAAAGCTATCAACACTCTGAATGGATTGAGACTTCAAACCAAAACAATAAAAGTTTCCTATGCTCGCCC
AAGCTCAGCCTCAATCAGAGATGCAAACTTATACGTCAGCGGGCTTCCAAAAACCATGACCCAGAAGGAG
CTGGAACAGCTGTTTTCACAGTATGGGCGCATCATTACTTCTCGGATTCTTGTCGACCAGGTCACTGGCA
TATCAAGGGGTGTAGGGTTTATTCGATTTGACAAGCGGATTGAGGCAGAAGAAGCTATCAAAGGCCTAAA
TGGCCAGAAACCTCCTGGTGCCACAGAGCCAATCACTGTAAAGTTTGCTAATAATCCAAGCCAAAAAACC
AATCAGGCCATCCTTTCCCAGCTGTACCAGTCTCCAAACAGAAGGTACCCAGGACCGCTAGCTCAGCAGG
CACAGCGTTTTAGTAGGTTTTCTCCAATGACGATCGATGGAATGACCAGTCTGGCTGGAATTAATATTCC
TGGGCACCCTGGAACAGGCTGGTGCATATTTGTGTACAACCTGGCGCCTGATGCAGATGAAAGTATCCTG
TGGCAAATGTTTGGGCCTTTTGGAGCTGTCACCAACGTGAAGGTCATCCGTGACTTTAACACCAATAAGT
GCAAAGGTTTTGGATTTGTGACTATGACAAACTATGATGAGGCGGCCATGGCGATAGCTAGCCTCAATGG
ATACCGCCTGGGAGACAGAGTCCTGCAGGTCTCCTTTAAGACAAACAAAACGCACAAAGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205233 representing BC046598
Red=Cloning site Green=Tags(s)

METQLSNGPTCNNTANGPTTVNNNCSSPVDSGNTEDSKTNLIVNYLPQNMTQEELKSLFGSIGEIESCKL
VRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKE
LEQLFSQYGRIITSRILVDQVTGISRGVGFIRFDKRIEAEEAIKGLNGQKPPGATEPITVKFANNPSQKT
NQAILSQLYQSPNRRYPGPLAQQAQRFSRFSPMTIDGMTSLAGINIPGHPGTGWCIFVYNLAPDADESIL
WQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGDRVLQVSFKTNKTHKA

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC046598
ORF Size 1041 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC046598, AAH46598
RefSeq Size 3665 bp
RefSeq ORF 1043 bp
Locus ID 15569
Cytogenetics 4 42.36 cM
Summary Binds RNA. Seems to recognize a GAAA motif. Can bind to its own 3' UTR, the FOS 3' UTR and the ID 3' UTR.UniProtKB/Swiss-Prot Function
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Documents
Resources

Plasmid Map for MG205233

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG205233
Circular map for MG205233

file-alt Citations

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