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Duoxa1 (NM_145395) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG205128
Duoxa1 (tGFP-tagged) - Mouse dual oxidase maturation factor 1 (Duoxa1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Duoxa1
Synonyms BC019755; Nip; Nip1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205128 representing NM_145395
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTCTTGGACACACACTGCCCTTCTACACTGGCACCAAGCCAACCTTTCCAATGGACACTACTC
TGGCTGTCATTATCACCATCTTCCTGACTGCACTGGTCACCTTCATCATTATCCTGCCTGGCATCCGTGG
GAAGACGAGGCTGTTCTGGCTGCTTCGGGTGGTGACCAGCTTATTCATCGGGGCTGTGATCCTGGCTGTG
AACTTCAGTTCTGAGTGGTCTGTGGGCCATGTCAACGCCAACACAACGTACAAAGCCTTCAGTCCCAAGT
GGGTCAGTGTGGACGTGGGACTGCAGATCGGGCTCGGCGGCGTCAACATCACCTTCACAGGCACCCCAGT
GCAGCAGCTGAATGAAACCATCAACTACAATGAGGCATTCGCATGGCGCCTGGGCAGGAGCTATGCAGAG
GAGTATGCAAAGGCCCTGGAGAAGGGCCTTCCAGACCCAGTGCTATACCTGGCTGAGAAGTTCACCCCTC
GAAGCCCATGTGGCCTGTACAACCAGTACCGCCTGGCAGGACACTATGCCTCTGCCATGCTGTGGGTGGC
ATTCCTCTGCTGGCTGCTGGCCAATGTGATGTTGTCGATGCCAGTGCTGGTTTATGGTGGCCACATGCTG
CTGGCCACTGGTCTCTTCCAGCTGTTGGCCCTGTTCTTCTTCTCCATGACCACGTCACTCATATCGCCCT
GTCCCCTGCGCTTGGGCACTGCTGTGCTGCACACTCACCATGGGCCCGCTTTCTGGATCACGTTAGCCAC
AGGACTGCTGTGTATTCTGCTTGGCCTGGTCATGGCAGTGGCCCACAGGATGCAGCCTCACAGACTGAAG
GCTTTCTTCAACCTGAGTTCAGAGGACCCAGTGCTGGAGTGGGGCTCTGAAGAAGGAGGACTCTTGAGCC
CCCACTACCGGTCCATAGCTGAGAGTCCTGAGACCCAGGACACTCCCATGTCAGTGGCTTCCTCTGAGAC
ATGTTTTAAGGAGGAACACCCCAAAGAGTCCGACTGTTCCCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205128 representing NM_145395
Red=Cloning site Green=Tags(s)

MAALGHTLPFYTGTKPTFPMDTTLAVIITIFLTALVTFIIILPGIRGKTRLFWLLRVVTSLFIGAVILAV
NFSSEWSVGHVNANTTYKAFSPKWVSVDVGLQIGLGGVNITFTGTPVQQLNETINYNEAFAWRLGRSYAE
EYAKALEKGLPDPVLYLAEKFTPRSPCGLYNQYRLAGHYASAMLWVAFLCWLLANVMLSMPVLVYGGHML
LATGLFQLLALFFFSMTTSLISPCPLRLGTAVLHTHHGPAFWITLATGLLCILLGLVMAVAHRMQPHRLK
AFFNLSSEDPVLEWGSEEGGLLSPHYRSIAESPETQDTPMSVASSETCFKEEHPKESDCSL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145395
ORF Size 1023 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145395.1
RefSeq Size 1513 bp
RefSeq ORF 1026 bp
Locus ID 213696
Cytogenetics 2 E5
Summary May be required for the maturation and the transport from the endoplasmic reticulum to the plasma membrane of functional DUOX1.UniProtKB/Swiss-Prot Function
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Documents
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"NM_145395" in other vectors (4)
SKU Description Size Price
MC201449 Duoxa1 (untagged) - Mouse dual oxidase maturation factor 1 (Duoxa1), (10ug) 10 ug
$457.00
MR205128 Duoxa1 (Myc-DDK-tagged) - Mouse dual oxidase maturation factor 1 (Duoxa1) 10 ug
$289.00 $457.00
MR205128L3 Lenti ORF clone of Duoxa1 (Myc-DDK-tagged) - Mouse dual oxidase maturation factor 1 (Duoxa1) 10 ug
$757.00
MR205128L4 Lenti ORF clone of Duoxa1 (mGFP-tagged) - Mouse dual oxidase maturation factor 1 (Duoxa1) 10 ug
$757.00

Plasmid Map for MG205128

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG205128
Circular map for MG205128

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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