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Gnb2 (NM_010312) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG205096
Gnb2 (tGFP-tagged) - Mouse guanine nucleotide binding protein, beta 2 (Gnb2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gnb2
Synonyms Gnb-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205096 representing NM_010312
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGAGCTGGAGCAACTGAGACAGGAGGCCGAGCAGCTCCGGAACCAGATCCGGGACGCCAGAAAAG
CATGCGGGGATTCCACACTGACCCAGATCACAGCTGGGCTGGACCCAGTGGGGCGAATTCAGATGAGAAC
ACGGAGGACCCTCCGTGGACACCTGGCAAAAATCTATGCCATGCACTGGGGGACAGACTCAAGGCTGCTG
GTCAGCGCCTCCCAGGACGGAAAGCTCATCATTTGGGACAGCTACACCACTAACAAGGTCCACGCCATCC
CTCTGCGTTCCTCCTGGGTCATGACCTGTGCCTACGCCCCCTCAGGGAACTTTGTGGCCTGTGGGGGTTT
GGACAACATCTGCTCCATCTATAGTCTCAAGACCCGAGAGGGCAATGTCAGGGTCAGCAGAGAGCTGCCT
GGCCACACTGGGTACCTGTCATGCTGCCGCTTCCTGGACGACAACCAAATCATCACCAGCTCTGGGGACA
CCACCTGTGCCCTGTGGGACATTGAGACAGGCCAGCAGACGGTGGGGTTTGCTGGACACAGTGGGGATGT
GATGTCCCTGTCACTGGCCCCCGATGGCCGCACCTTTGTGTCAGGTGCCTGTGACGCCTCCATCAAGCTG
TGGGATGTTCGGGATTCCATGTGCCGACAGACATTCATAGGTCACGAGTCGGACATCAATGCCGTGGCTT
TCTTCCCCAATGGCTATGCCTTCACCACGGGATCAGATGACGCCACTTGTCGCCTCTTTGACCTGCGGGC
TGATCAGGAGCTGCTCATGTATTCCCACGACAACATCATCTGCGGCATCACCTCGGTTGCCTTCTCGCGC
AGCGGCCGGCTGCTGCTCGCTGGCTACGACGACTTCAACTGCAACATCTGGGATGCCATGAAGGGTGACC
GTGCAGGTGTCCTCGCTGGCCATGATAACCGTGTGAGCTGCCTTGGGGTCACAGATGATGGAATGGCCGT
GGCTACAGGCTCCTGGGACTCCTTCCTCAAGATCTGGAAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205096 representing NM_010312
Red=Cloning site Green=Tags(s)

MSELEQLRQEAEQLRNQIRDARKACGDSTLTQITAGLDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLL
VSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNFVACGGLDNICSIYSLKTREGNVRVSRELP
GHTGYLSCCRFLDDNQIITSSGDTTCALWDIETGQQTVGFAGHSGDVMSLSLAPDGRTFVSGACDASIKL
WDVRDSMCRQTFIGHESDINAVAFFPNGYAFTTGSDDATCRLFDLRADQELLMYSHDNIICGITSVAFSR
SGRLLLAGYDDFNCNIWDAMKGDRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010312
ORF Size 1020 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010312.5
RefSeq Size 1619 bp
RefSeq ORF 1023 bp
Locus ID 14693
UniProt ID P62880
Cytogenetics 5 76.54 cM
Summary Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.UniProtKB/Swiss-Prot Function
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"NM_010312" in other vectors (4)
SKU Description Size Price
MC204625 Gnb2 (untagged) - Mouse guanine nucleotide binding protein (G protein), beta 2 (Gnb2), (10ug) 10 ug
$457.00
MR205096 Gnb2 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), beta 2 (Gnb2) 10 ug
$289.00 $457.00
MR205096L3 Lenti ORF clone of Gnb2 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), beta 2 (Gnb2) 10 ug
$757.00
MR205096L4 Lenti ORF clone of Gnb2 (mGFP-tagged) - Mouse guanine nucleotide binding protein (G protein), beta 2 (Gnb2) 10 ug
$757.00

Plasmid Map for MG205096

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG205096
Circular map for MG205096

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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