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Dcps (NM_027030) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG205053
Dcps (tGFP-tagged) - Mouse decapping enzyme, scavenger (Dcps)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dcps
Synonyms 1700001E16Rik; AA408441
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205053 representing NM_027030
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGATACAGCGCCTCAACTCAAGAGAAAGCGCGAACAGGAGGCAGAGGAGGCAGAAACCCCCAGCA
CAGAGGAGAAGGAAGCAGGCGTTGGCAATGGCACCTCTGCCCCTGTCCGCTTACCGTTCTCCGGCTTTAG
AGTACAAAAAGTGCTCAGGGAGTCTGCGCGGGACAAAATTATTTTCCTGCATGGGAAGGTGAATGAGGAC
TCTGGGGATACTCATGGAGAAGATGCGGTTGTGATCCTGGAGAAGACACCATTTCAGGTAGAACACGTGG
CGCAGCTCCTAACGGGGAGCCCTGAGCTCAAGTTGCAGTTCTCCAATGATATCTACAGCACCTATAACCT
GTTTCCTCCAAGGCATCTGAGTGATATAAAAACAACTGTGGTGTACCCTGCCACAGAGAAACACCTGCAA
AAATACATGCGTCAGGACCTCCGCCTGATCCGAGAGACTGGAGATGACTACAGGACCATCACCTTACCCT
ACCTGGAATCCCAGAGCCTTAGCATCCAGTGGGTGTATAACATTCTTGACAAGAAGGCTGAAGCTGACCG
GATTGTTTTTGAGAACCCAGACCCTTCTGATGGCTTTGTCCTCATCCCAGACCTCAAGTGGAACCAGCAG
CAGCTTGATGACCTGTATTTGATCGCCATCTGCCATCGCCGGGGTATCAGATCACTTCGAGATCTCACTC
CAGAGCATCTGCCACTACTGAGGAACATTCTCCGGGAAGGACAAGAAGCCATCCTGAAGCGCTACCAGGT
GACAGGAGACCGTCTGCGAGTGTACCTACACTACCTGCCCTCTTACTATCACCTGCACGTGCATTTCACA
GCTCTGGGCTTCGAGGCTCCGGGCTCAGGGGTGGAGCGGGCACACCTGCTGGCTCAAGTGATCGAGAACC
TGGAGTGTGACCCCAAGCACTATCAACAGCGCACTCTTACTTTTGCCCTCAGGACCGATGACCCCCTGCT
TCAGCTCCTGCAGAAGGCCCAGCAAGAGAGGAAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205053 representing NM_027030
Red=Cloning site Green=Tags(s)

MADTAPQLKRKREQEAEEAETPSTEEKEAGVGNGTSAPVRLPFSGFRVQKVLRESARDKIIFLHGKVNED
SGDTHGEDAVVILEKTPFQVEHVAQLLTGSPELKLQFSNDIYSTYNLFPPRHLSDIKTTVVYPATEKHLQ
KYMRQDLRLIRETGDDYRTITLPYLESQSLSIQWVYNILDKKAEADRIVFENPDPSDGFVLIPDLKWNQQ
QLDDLYLIAICHRRGIRSLRDLTPEHLPLLRNILREGQEAILKRYQVTGDRLRVYLHYLPSYYHLHVHFT
ALGFEAPGSGVERAHLLAQVIENLECDPKHYQQRTLTFALRTDDPLLQLLQKAQQERN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027030
ORF Size 1014 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027030.2
RefSeq Size 1195 bp
RefSeq ORF 1017 bp
Locus ID 69305
UniProt ID Q9DAR7
Cytogenetics 9 A4
Summary Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP). Cleaves cap analog structures like tri-methyl guanosine nucleoside triphosphate (m3(2,2,7)GpppG) with very poor efficiency. Does not hydrolyze unmethylated cap analog (GpppG) and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7-methylguanosine diphosphate (m7GDP) to m7GMP. May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCPS to m7GMP. Binds to m7GpppG and strongly to m7GDP. Plays a role in first intron splicing of pre-mRNAs. Inhibits activation-induced cell death.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027030" in other vectors (4)
SKU Description Size Price
MC203955 Dcps (untagged) - Mouse decapping enzyme, scavenger (Dcps), (10ug) 10 ug
$457.00
MR205053 Dcps (Myc-DDK-tagged) - Mouse decapping enzyme, scavenger (Dcps) 10 ug
$289.00 $457.00
MR205053L3 Lenti ORF clone of Dcps (Myc-DDK-tagged) - Mouse decapping enzyme, scavenger (Dcps) 10 ug
$757.00
MR205053L4 Lenti ORF clone of Dcps (mGFP-tagged) - Mouse decapping enzyme, scavenger (Dcps) 10 ug
$757.00

Plasmid Map for MG205053

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG205053
Circular map for MG205053

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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