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Hsh2d (NM_197944) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG204940
Hsh2d (tGFP-tagged) - Mouse hematopoietic SH2 domain containing (Hsh2d)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hsh2d
Synonyms ALX; Hsh2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG204940 representing NM_197944
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAAGCCAGGAGGCTGCCCCCACCACTACCTCCTCGGCTGGACTGGTTTGTGCACACCCAGGCGG
ACCTGCTAGCCCAATCCGGGATCCCCGAGTGGTTCCATGGCACCATCTCCCGAGAGGCTGCTGAGAACAT
GTTGGAGTCACAACCCCTGGGAACTTTTCTTATCCGAGTCAGCCACAGCCATGTGGGCTACACCCTTTCC
TACAAAGCCCAGACTTGCTGCCGTCACTTCATGGTGAAACTTTCAGAAGATGGGACTTGCACCTTCGCTG
GGGACCACATGACCCACGCCTCTCTGCATGCCCTGGTCACCTTCCACCAACAAAAGCCTATTCGGCCATT
CGGGGAGCTGTTGACCCAGGCCTGCGGGCAGGAGGATCCAGCAAACGTGGACTATGAAGATCTCTTCCTA
TACTCAAATGCCCTGGTTCAGGATGCTGAAAGCCAATTTCTGAGAACGGAAGTTCAAAGGTCTTCTTCCT
GCCCACCTGAGGAGGCTTCTGAAAGGAAGCCATCCACAACAACTAAAGGAGAATTCGCATCAGCTTCCTG
CTCCCCAAAAGCCCTTTTCGAGGATTCTGGACAGAAACTGTGGAAGAACCTGAGGTCCCTACCTCAGACT
AGCCAGAGGGTGAAACAGCAACTCACTTCACACCTGTCGGCCATGAACCTGCTGGGGGATGCCAGGCGAG
TGGCACAGCAGCACCACAGCCCTGTGACTCGAGCATTCAGTTGGGACAGTACTAGCCACTCCGAGGACCC
CTGTGCAGCCACCACGTCTCTTCAAAACCCTGCAGAACCCCAGGCCCTGAGAGGCAGAGAAGCCACCTTC
AGGGACTCCAGGCCTGCCAGCTGGAGGAAGGCATTCTCAGGGATCAAAGCCTGGCGTGGAAAGGTGGTAA
GGGCCCTGTCAGCCCAGGAGCCTGTGGACTTTCCAGAAGCCCAGGGCTGGTTGCCTGAGGAATACCTTCC
ACCACCACCTTTCGCCCCTGGGTAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG204940 representing NM_197944
Red=Cloning site Green=Tags(s)

MAEARRLPPPLPPRLDWFVHTQADLLAQSGIPEWFHGTISREAAENMLESQPLGTFLIRVSHSHVGYTLS
YKAQTCCRHFMVKLSEDGTCTFAGDHMTHASLHALVTFHQQKPIRPFGELLTQACGQEDPANVDYEDLFL
YSNALVQDAESQFLRTEVQRSSSCPPEEASERKPSTTTKGEFASASCSPKALFEDSGQKLWKNLRSLPQT
SQRVKQQLTSHLSAMNLLGDARRVAQQHHSPVTRAFSWDSTSHSEDPCAATTSLQNPAEPQALRGREATF
RDSRPASWRKAFSGIKAWRGKVVRALSAQEPVDFPEAQGWLPEEYLPPPPFAPGY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_197944
ORF Size 1005 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_197944.1, NP_922935.1
RefSeq Size 1225 bp
RefSeq ORF 1005 bp
Locus ID 209488
UniProt ID Q6VYH9
Cytogenetics 8 B3.3
Summary Adapter protein involved in tyrosine kinase and CD28 signaling (By similarity). May be a modulator of the apoptotic response through its ability to affect mitochondrial stability.UniProtKB/Swiss-Prot Function
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"NM_197944" in other vectors (4)
SKU Description Size Price
MC212524 Hsh2d (untagged) - Mouse hematopoietic SH2 domain containing (Hsh2d), (10ug) 10 ug
$503.00
MR204940 Hsh2d (Myc-DDK-tagged) - Mouse hematopoietic SH2 domain containing (Hsh2d) 10 ug
$289.00 $457.00
MR204940L3 Lenti ORF clone of Hsh2d (Myc-DDK-tagged) - Mouse hematopoietic SH2 domain containing (Hsh2d) 10 ug
$757.00
MR204940L4 Lenti ORF clone of Hsh2d (mGFP-tagged) - Mouse hematopoietic SH2 domain containing (Hsh2d) 10 ug
$757.00

Plasmid Map for MG204940

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG204940
Circular map for MG204940

file-alt Citations

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