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Eif3i (NM_018799) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG204729
Eif3i (tGFP-tagged) - Mouse eukaryotic translation initiation factor 3, subunit 2 (beta) (Eif3s2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Eif3i
Synonyms 36kDa; D4Ertd632e; Eif3s2; Trip1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG204729 representing NM_018799
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAACCCATCCTGCTGCAGGGCCATGAGCGGTCCATCACGCAGATCAAGTATAACCGCGAAGGAGACC
TCCTCTTCACCGTGGCCAAAGACCCTATCGTCAACGTGTGGTACTCTGTCAATGGGGAGAGGCTAGGCAC
CTACATGGGCCACACTGGAGCTGTGTGGTGCGTGGATGCTGACTGGGACACCAAGCATGTCCTTACTGGC
TCAGCTGACAACAGCTGTCGTCTCTGGGACTGTGAAACAGGGAAGCAGCTGGCCCTACTCAAGACCAACT
CAGCTGTCCGGACCTGCGGCTTTGACTTTGGGGGCAACATCATCATGTTCTCCACAGACAAGCAGATGGG
GTATCAGTGCTTTGTGAGCTTCTTTGATCTGCGGGATCCAAGCCAGATCGACAGCAACGAGCCCTACATG
AAGATCCCCTGTAATGATTCCAAGATCACCAGTGCCGTCTGGGGCCCCCTCGGGGAGTGCGTCATCGCAG
GCCACGAGAGCGGAGAGCTCAACCAGTATAGCGCCAAGTCTGGAGAGGTGTTGGTGAACGTAAAGGAACA
CTCTCGGCAGATCAATGACATCCAGTTGTCTAGAGACATGACCATGTTTGTCACTGCATCTAAGGACAAC
ACAGCTAAGCTCTTCGACTCCACGACTCTTGAACATCAGAAGACTTTCCGAACAGAGCGTCCTGTCAACT
CGGCTGCTCTCTCTCCCAACTATGACCATGTGGTGCTGGGAGGAGGTCAGGAAGCCATGGATGTGACCAC
AACCTCCACCAGGATTGGCAAGTTTGAGGCCAGGTTCTTCCATTTGGCTTTTGAGGAAGAGTTTGGAAGA
GTCAAGGGCCACTTTGGACCGATCAACAGCGTTGCCTTCCATCCTGATGGCAAGAGCTACAGCAGCGGTG
GCGAAGATGGTTACGTCCGCATCCACTACTTCGACCCACAGTACTTTGAGTTCGAGTTTGAGGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG204729 representing NM_018799
Red=Cloning site Green=Tags(s)

MKPILLQGHERSITQIKYNREGDLLFTVAKDPIVNVWYSVNGERLGTYMGHTGAVWCVDADWDTKHVLTG
SADNSCRLWDCETGKQLALLKTNSAVRTCGFDFGGNIIMFSTDKQMGYQCFVSFFDLRDPSQIDSNEPYM
KIPCNDSKITSAVWGPLGECVIAGHESGELNQYSAKSGEVLVNVKEHSRQINDIQLSRDMTMFVTASKDN
TAKLFDSTTLEHQKTFRTERPVNSAALSPNYDHVVLGGGQEAMDVTTTSTRIGKFEARFFHLAFEEEFGR
VKGHFGPINSVAFHPDGKSYSSGGEDGYVRIHYFDPQYFEFEFEA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018799
ORF Size 975 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018799.1, NP_061269.1
RefSeq Size 1114 bp
RefSeq ORF 978 bp
Locus ID 54709
UniProt ID Q9QZD9
Cytogenetics 4 63.26 cM
Summary Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.UniProtKB/Swiss-Prot Function
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"NM_018799" in other vectors (4)
SKU Description Size Price
MC204531 Eif3i (untagged) - Mouse eukaryotic translation initiation factor 3, subunit I (Eif3i), (10ug) 10 ug
$300.00
MR204729 Eif3i (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 3, subunit I (Eif3i) 10 ug
$289.00 $300.00
MR204729L3 Lenti ORF clone of Eif3i (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 3, subunit I (Eif3i) 10 ug
$600.00
MR204729L4 Lenti ORF clone of Eif3i (mGFP-tagged) - Mouse eukaryotic translation initiation factor 3, subunit I (Eif3i) 10 ug
$600.00

Plasmid Map for MG204729

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG204729
Circular map for MG204729

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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