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Aida (NM_181732) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG204280
Aida (tGFP-tagged) - Mouse RIKEN cDNA 2610208M17 gene (2610208M17Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Aida
Synonyms 2610208M17Rik; BC004835
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG204280 representing NM_181732
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGAGGTGACCCGGAGTCTGCTGCAGCGCTGGGGCGCCAGTCTTAGGAGAGGCGCCGACTTCGACT
CTTGGGGCCAGCTGGTGGAGGCGATAGACGAGTATCAGATATTAGCAAGACACTTACAGAAGGAGGCCCA
AGCTCAACACAATAATTCTGAATTTACAGAAGAACAAAAGAAAACCATAGGCAAAATTGCGACATGTTTG
GAATTGCGAAGTGCAGCATTACAGTCCACACAGTCCCAAGAAGAGTTTAAACTGGAGGACCTGAAGAAGC
TAGAACCAATCCTGAAGAATATTCTTACATATAATAAAGAATTCCCATTTGATGTTCAGCCTATTCCATT
AAGACGTATTTTAGCACCTGGTGAAGAAGAGAATTTGGAATTTGAAGAAGATGAAGAAGGTGGTGCTGGA
GCAGGGCCTCCTGATTCTTTCTCTGCTAGAGTTCCTGGTACTTTATTGCCACGGTTGCCATCGGAACCAG
GGATGACGTTACTCACCATCAGGATTGAGAAAATTGGTTTGAAAGATGCGGGGCAGTGCATCGATCCCTA
CATCACAGTCAGCGTGAAAGATTTGAATGGCATAGATTTAACTCCCGTACAAGACACTCCTGTGGCTTCA
AGGAAAGAAGATACTTACGTTCACTTTAATGTGGACATTGAGCTCCAGAAACACGTTGAAAGGTTAACCA
AAGGTGCGGCCATTTTCTTTGAATTCAAACATTACAAGCCTAAAAAGAGGTTTACCAGCACTAAGTGTTT
TGCCTTCATGGAGATGGATGAAATTAAACCTGGGCCAATCGTAATAGAACTATACAAGAAACCCACTGAC
TTTAAAAGAAAGAAATTGCAATTGTTGACCAAGAAACCACTTTATCTTCATCTACATCAAAGTTTGCACA
AGGAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG204280 representing NM_181732
Red=Cloning site Green=Tags(s)

MSEVTRSLLQRWGASLRRGADFDSWGQLVEAIDEYQILARHLQKEAQAQHNNSEFTEEQKKTIGKIATCL
ELRSAALQSTQSQEEFKLEDLKKLEPILKNILTYNKEFPFDVQPIPLRRILAPGEEENLEFEEDEEGGAG
AGPPDSFSARVPGTLLPRLPSEPGMTLLTIRIEKIGLKDAGQCIDPYITVSVKDLNGIDLTPVQDTPVAS
RKEDTYVHFNVDIELQKHVERLTKGAAIFFEFKHYKPKKRFTSTKCFAFMEMDEIKPGPIVIELYKKPTD
FKRKKLQLLTKKPLYLHLHQSLHKE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181732
ORF Size 915 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181732.4
RefSeq Size 2970 bp
RefSeq ORF 918 bp
Locus ID 108909
UniProt ID Q8C4Q6
Cytogenetics 1 H5
Summary Acts as a ventralizing factor during embryogenesis (By similarity). Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.UniProtKB/Swiss-Prot Function
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"NM_181732" in other vectors (3)
SKU Description Size Price
MR204280 Aida (Myc-DDK-tagged) - Mouse axin interactor, dorsalization associated (Aida) 10 ug
$289.00 $300.00
MR204280L3 Lenti ORF clone of Aida (Myc-DDK-tagged) - Mouse axin interactor, dorsalization associated (Aida) 10 ug
$600.00
MR204280L4 Lenti ORF clone of Aida (mGFP-tagged) - Mouse axin interactor, dorsalization associated (Aida) 10 ug
$600.00

Plasmid Map for MG204280

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MG204280
Circular map for MG204280

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