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Dtwd1 (NM_026981) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG204261
Dtwd1 (tGFP-tagged) - Mouse DTW domain containing 1 (Dtwd1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dtwd1
Synonyms 1810033A06Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG204261 representing NM_026981
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTCAGTCCATCTGTAGTTCCTCAAGAAAGTAAAGAAGATAATGCAAACTGTGTAGAAACAAAAC
CGTCACAAACCACTTCCATTGCTTCAGAAGACCCCCTTCAAAACTTATGCTTAGCGTCTCAGGAAGTGCT
GCGTAAGGCTCAACAAAGTGGGAGGTCCCGGTGTCGCCAATGTGGTGGCTCAAGGATGTTCTACTGCTAC
ACATGCTGTGTCCCAGTGGGAAACGTGCCCACCGAGCAGATCCCATGTGTGCAGCTTCCATTGAAGATTG
ATATCATTAAGCATCCAAATGAAACAGACGGCAAGAGCACCGCTGTGCACGCAAAGCTCTTAGCGCCCGA
CTCTGTGAACATTTACACATATCCGTGCATTCCGGAGTACGAAGGGAAGGATCACGAGGTTGTACTTGTT
TTTCCTGGGCCTCAGTCTATCTCAATAGAAGATGTCTCTTTCCATCTACAAAAAAGGATTGAAAGTAAGG
GTAGAAACAAAGCTGACAACCTTGATGTGCCACCTCGGAAACTCAAGAGGACTACGGACGAGGAAGGCTG
GGATCTGCATGAGAGCACACGCCAAGGCCCCGAGTTGAAAAGAGTTGTCTTTATTGACAGCACCTGGAGC
CAGACAAACCAGATCGCCTCTGATGAGCGGCTTCGGGAGTTATTACAAGTTGAACTGAGAACAAGAAAAA
CTTGCTTTTGGCGCCATCAAAAAGGGAAGCCAGATACTTTCCTTTCCACAATTGAAGCCATTTATTACTT
CCTAGTAGACTACCATAGTGCCGTGCAGAAAGAGAAGTACAGAGGACAGTATGACAACCTTTTATTCTTC
TACTCTTTTATGTACCGCTTGATAAAGAATGCCAGAGGCTCTGGAGAGAAGGCTAAACCGCAGCTCGTCC
AG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG204261 representing NM_026981
Red=Cloning site Green=Tags(s)

MALSPSVVPQESKEDNANCVETKPSQTTSIASEDPLQNLCLASQEVLRKAQQSGRSRCRQCGGSRMFYCY
TCCVPVGNVPTEQIPCVQLPLKIDIIKHPNETDGKSTAVHAKLLAPDSVNIYTYPCIPEYEGKDHEVVLV
FPGPQSISIEDVSFHLQKRIESKGRNKADNLDVPPRKLKRTTDEEGWDLHESTRQGPELKRVVFIDSTWS
QTNQIASDERLRELLQVELRTRKTCFWRHQKGKPDTFLSTIEAIYYFLVDYHSAVQKEKYRGQYDNLLFF
YSFMYRLIKNARGSGEKAKPQLVQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026981
ORF Size 912 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026981.2
RefSeq Size 1309 bp
RefSeq ORF 915 bp
Locus ID 69185
UniProt ID Q9D8U7
Cytogenetics 2 F1
Summary Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine (acp3U) at position 20 in the D-loop of several cytoplasmic tRNAs (acp3U(20)).UniProtKB/Swiss-Prot Function
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"NM_026981" in other vectors (4)
SKU Description Size Price
MC203911 Dtwd1 (untagged) - Mouse DTW domain containing 1 (Dtwd1), (10ug) 10 ug
$300.00
MR204261 Dtwd1 (Myc-DDK-tagged) - Mouse DTW domain containing 1 (Dtwd1) 10 ug
$289.00 $300.00
MR204261L3 Lenti ORF clone of Dtwd1 (Myc-DDK-tagged) - Mouse DTW domain containing 1 (Dtwd1) 10 ug
$600.00
MR204261L4 Lenti ORF clone of Dtwd1 (mGFP-tagged) - Mouse DTW domain containing 1 (Dtwd1) 10 ug
$600.00

Plasmid Map for MG204261

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG204261
Circular map for MG204261

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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