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Dtwd1 (NM_026981) Mouse Untagged Clone

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SKU
MC203911
Dtwd1 (untagged) - Mouse DTW domain containing 1 (Dtwd1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dtwd1
Synonyms 1810033A06Rik
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC020149
CGAGAGTTGAGCGTGGTGCAGTGTACCTATGATACATGCTTTAGAAACAGAATATTTTGGTTCAAAGGGA AAATGGCTCTCAGTCCATCTGTAGTTCCTCAAGAAAGTAAAGAAGATAATGCAAACTGTGTAGAAACAAA ACCGTCACAAACCACTTCCATTGCTTCAGAAGACCCCCTTCAAAACTTATGCTTAGCGTCTCAGGAAGTG CTGCGTAAGGCTCAACAAAGTGGGAGGTCCCGGTGTCGCCAATGTGGTGGCTCAAGGATGTTCTACTGCT ACACATGCTGTGTCCCAGTGGGAAACGTGCCCACCGAGCAGATCCCATGTGTGCAGCTTCCATTGAAGAT TGATATCATTAAGCATCCAAATGAAACAGACGGCAAGAGCACCGCTGTGCACGCAAAGCTCTTAGCGCCC GACTCTGTGAACATTTACACATATCCGTGCATTCCGGAGTACGAAGGGAAGGATCACGAGGTTGTACTTG TTTTTCCTGGGCCTCAGTCTATCTCAATAGAAGATGTCTCTTTCCATCTACAAAAAAGGATTGAAAGTAA GGGTAGAAACAAAGCTGACAACCTTGATGTGCCACCTCGGAAACTCAAGAGGACTACGGACGAGGAAGGC TGGGATCTGCATGAGAGCACACGCCAAGGCCCCGAGTTGAAAAGAGTTGTCTTTATTGACAGCACCTGGA GCCAGACAAACCAGATCGCCTCTGATGAGCGGCTTCGGGAGTTATTACAAGTTGAACTGAGAACAAGAAA AACTTGCTTTTGGCGCCATCAAAAAGGGAAGCCAGATACTTTCCTTTCCACAATTGAAGCCATTTATTAC TTCCTAGTAGACTACCATAGTGCCGTGCAGAAAGAGAAGTACAGAGGACAGTATGACAACCTTTTATTCT TCTACTCTTTTATGTACCGCTTGATAAAGAATGCCAGAGGCTCTGGAGAGAAGGCTAAACCGCAGCTCGT CCAGTAGTTCTGCACACCCGCTTGTCCTGGGCTCTTGCAGTCGAGCCTTGTTTACTCCGGGGTGGTAACC ATGTGTTATGCCCATGAGAGAACATGGGGAAGATTCCAGCCTTGTTCGTTTTATTGTATTTTTTTAAAGG GAATTTTGAATTTGGGCAATTTTTAAGAGATGATCCTGATTGAAAGACAAATCAGAAGCTATTTGCTCAA TTTGGGCATATCCCACTATACTGTACATTTTAATATGCTGTGTTACATTTATGTGATTGTAATTATATGT GATTAAAGAGTGTCTTTATCTTTTCACTTAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_026981
Insert Size 915 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC020149, AAH20149
RefSeq Size 1312 bp
RefSeq ORF 915 bp
Locus ID 69185
UniProt ID Q9D8U7
Cytogenetics 2 F1
Summary Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine (acp3U) at position 20 in the D-loop of several cytoplasmic tRNAs (acp3U(20)).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.
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Documents
Resources
"NM_026981" in other vectors (4)
SKU Description Size Price
MG204261 Dtwd1 (tGFP-tagged) - Mouse DTW domain containing 1 (Dtwd1) 10 ug
$500.00
MR204261 Dtwd1 (Myc-DDK-tagged) - Mouse DTW domain containing 1 (Dtwd1) 10 ug
$289.00 $300.00
MR204261L3 Lenti ORF clone of Dtwd1 (Myc-DDK-tagged) - Mouse DTW domain containing 1 (Dtwd1) 10 ug
$600.00
MR204261L4 Lenti ORF clone of Dtwd1 (mGFP-tagged) - Mouse DTW domain containing 1 (Dtwd1) 10 ug
$600.00

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