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Ankrd54 (NM_144849) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG204172
Ankrd54 (tGFP-tagged) - Mouse ankyrin repeat domain 54 (Ankrd54)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ankrd54
Synonyms C730048E16Rik; EST475269; EST1068184; Liar
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG204172 representing NM_144849
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCACCGGCGGGGGCGCGGACGACGAGTCGCGATCCGGCCGCTCGAGCTCGGACGGCGAGTGCG
CGGTGGCGCCGGAGCCTTTGGCGGAAGCCGGAGGCCTGGTGTCTTTCGCGGACTTCGGCGTTTCGCTGGG
CAGCGGCGCCGGCCTCCCGGGCCGGTCGGTGGGCAGGGCCCAGTCCTCGCTGCGCTACCTCCAGGTCCTG
TGGCAGCAGGACGTCGAGCCTCGCGACGAACTGCGTTGCAAGATTCCCGCCGGGCGGTTGAGGCGTGCCG
CCAGGCCCCACCGTCGGCTCGGGCCCACCGGCAAGGAGGTACACGCTCTGAAGAGGCTGAGGGACTCTGC
CAATGCCAACGACGTAGAAACAGTGCAGCAGCTGCTGGAAGATGGGGCGGATCCCTGCGCAGCGGATGAT
AAAGGCAGAACTGCCCTCCATTTTGCTTCCTGCAATGGCAATGACCAGATTGTGCAGCTGCTCCTGGACC
ATGGGGCTGACCCCAACCAACAGGATGGTCTGGGCAACACGCCATTGCACCTGGCGGCCTGCACCAACCA
TGTTCCTGTCATCACCACACTGCTTCGAGGAGGGGCCCGTGTAGATGCCCTAGACAGAGCTGGCCGCACA
CCCCTACACCTGGCCAAGTCGAAGCTGAACATCCTACAGGAGGGTCATTCCCAGTGTCTGGAGGCCGTCC
GCCTGGAGGTGAAGCAGATCATCCACATGCTACGCGAGTACCTGGAGCGCCTGGGGCGGCATGAGCAGCG
AGAACGGCTAGATGACCTCTGCACCCGCCTCCAGATGACAAGTACCAAAGAACAGGTGGATGAAGTGACG
GACCTCTTGGCCAGCTTCACCTCCCTCAGTCTGCAGATGCAGAGTATGGAGAAGAGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG204172 representing NM_144849
Red=Cloning site Green=Tags(s)

MAATGGGADDESRSGRSSSDGECAVAPEPLAEAGGLVSFADFGVSLGSGAGLPGRSVGRAQSSLRYLQVL
WQQDVEPRDELRCKIPAGRLRRAARPHRRLGPTGKEVHALKRLRDSANANDVETVQQLLEDGADPCAADD
KGRTALHFASCNGNDQIVQLLLDHGADPNQQDGLGNTPLHLAACTNHVPVITTLLRGGARVDALDRAGRT
PLHLAKSKLNILQEGHSQCLEAVRLEVKQIIHMLREYLERLGRHEQRERLDDLCTRLQMTSTKEQVDEVT
DLLASFTSLSLQMQSMEKR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144849
ORF Size 897 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144849.1, NP_659098.1
RefSeq Size 1894 bp
RefSeq ORF 900 bp
Locus ID 223690
UniProt ID Q91WK7
Cytogenetics 15 37.7 cM
Summary Plays an important role in regulating intracellular signaling events associated with erythroid terminal differentiation.UniProtKB/Swiss-Prot Function
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"NM_144849" in other vectors (4)
SKU Description Size Price
MC201474 Ankrd54 (untagged) - Mouse ankyrin repeat domain 54 (Ankrd54), (10ug) 10 ug
$300.00
MR204172 Ankrd54 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 54 (Ankrd54) 10 ug
$289.00 $300.00
MR204172L3 Lenti ORF clone of Ankrd54 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 54 (Ankrd54) 10 ug
$600.00
MR204172L4 Lenti ORF clone of Ankrd54 (mGFP-tagged) - Mouse ankyrin repeat domain 54 (Ankrd54) 10 ug
$600.00

Plasmid Map for MG204172

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG204172
Circular map for MG204172

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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