Rpl6 (BC062880) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG204093
Rpl6 (tGFP-tagged) - Mouse ribosomal protein L6 (cDNA clone MGC:86071 IMAGE:5692572)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rpl6
Synonyms Taxreb107
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG204093 representing BC062880
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGTGAAAAAGCGCCTGATACAAAGGAGAAGAAACCTGCGGCCAAGAAGGCTGGCAGTGATGCTG
CTGCCTCCCGGCCCCGGGCCGCCAAAGTAGCCAAAAAGGTTCACCCTAAGGGTAAAAAGCCCAAGAAGGC
GAAGCCCCATTGCAGCCGAAACCCTGTCCTGGTGAGAGGAATTGGCAGGTACTCCCGATCTGCTATGTAC
TCCAGGAAGGCCTTTTACAAAAGGAAATACTCGGCTGCCAAGACCAAGGTTGAGAAGAAGAAGAAAAAAG
AGAAGGTCCTTGCTACTGTCACCAAAACAGTTGGTGGGGACAAGAACGGTGGCACCCGGGTGGTGAAGCT
TCGAAAAATGCCTAGGTATTATCCCACCGAAGATGTTCCTCGGAAGCTGCTGAGCCACGGCAAGAAGCCC
TTCAGCCAGCACGTGAGAAGGCTACGCTCCAGCATCACTCCCGGAACTGTCCTGATCATCCTCACTGGGC
GCCACAGGGGCAAGAGAGTGGTTTTCCTGAAGCAGCTGGACAGTGGCTTGCTGCTTGTGACTGGGCCTCT
TGTCATCAACAGAGTTCCTCTGCGCAGAACACACCAGAAGTTTGTCATTGCCACCTCTACAAAAGTTGAT
ATCAGCGATGTTAAAATCCCCAAACACCTGACTGACGCTTACTTCAAGAAGAAGCAGCTGCGCAAGCCCA
GGCATCAGGAGGGCGAGATCTTCGACACAGAGAAGGAGAAATACGAGATTACAGAGCAGCGAAAGGCTGA
CCAGAAAGCTGTGGATTTGCAGATTTTGCCAAAGATTAAAGCTGTTCCTCAGCTCCAGGGCTACCTGCGC
TCTCAGTTTTCCCTGACAAACGGGATGTATCCTCACAAACTGGTCTTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG204093 representing BC062880
Red=Cloning site Green=Tags(s)

MAGEKAPDTKEKKPAAKKAGSDAAASRPRAAKVAKKVHPKGKKPKKAKPHCSRNPVLVRGIGRYSRSAMY
SRKAFYKRKYSAAKTKVEKKKKKEKVLATVTKTVGGDKNGGTRVVKLRKMPRYYPTEDVPRKLLSHGKKP
FSQHVRRLRSSITPGTVLIILTGRHRGKRVVFLKQLDSGLLLVTGPLVINRVPLRRTHQKFVIATSTKVD
ISDVKIPKHLTDAYFKKKQLRKPRHQEGEIFDTEKEKYEITEQRKADQKAVDLQILPKIKAVPQLQGYLR
SQFSLTNGMYPHKLVF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC062880
ORF Size 888 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC062880.1
RefSeq Size 975 bp
RefSeq ORF 890 bp
Locus ID 19988
Cytogenetics 5 F
Summary Component of the large ribosomal subunit.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC062880" in other vectors (4)
SKU Description Size Price
MC218233 Rpl6 (untagged) - Mouse ribosomal protein L6 (cDNA clone MGC:86071 IMAGE:5692572), (10ug) 10 ug
$330.00
MR204093 Rpl6 (Myc-DDK-tagged) - Mouse ribosomal protein L6 (cDNA clone MGC:86071 IMAGE:5692572) 10 ug
$289.00 $300.00
MR204093L3 Lenti ORF clone of Rpl6 (Myc-DDK-tagged) - Mouse ribosomal protein L6 (cDNA clone MGC:86071 IMAGE:5692572) 10 ug
$600.00
MR204093L4 Lenti ORF clone of Rpl6 (mGFP-tagged) - Mouse ribosomal protein L6 (cDNA clone MGC:86071 IMAGE:5692572) 10 ug
$600.00

Plasmid Map for MG204093

Download sequence for

Cloning scheme for MG204093

Circular map for MG204093

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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