Pir (NM_027153) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG203965
Pir (tGFP-tagged) - Mouse pirin (Pir)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pir
Synonyms 2310042L19Rik; Pirnl
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG203965 representing NM_027153
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCCTCTAAAAAGGTTACTCTCTCAGTGCTCAGCCGGGAGCAGTCAGAGGGGGTAGGAGCACGTG
TGCGCAGAAGCATTGGCAGACCCGAGTTGAAAAATCTGGATCCATTTTTACTGTTTGATGAATTTAAAGG
AGGAAGGCCGGGAGGATTTCCTGATCATCCACACCGAGGATTTGAAACAGTATCTTATCTTCTGGAAGGG
GGCAGCATGGCCCATGAAGACTTTTGTGGACATGTTGGTAAAATGAACCCAGGAGACCTACAGTGGATGA
CTGCAGGACGAGGCATTCTGCATGCTGAGATGCCCTGCTCAGAGGAGCCAGCTCATGGCTTGCAGCTGTG
GGTGAATTTGAGACGCTCAGAAAAGATGGTGGCGCCTCAGTACCAGGAACTGAAAAGTGAAGAGATCCCG
AAACCCACCAAAGATGGTGTGACAGTTGCTGTCATTTCAGGAGAAGCCTTGGGAATAAAGTCGAAGGTTT
ACACTCGCACACCAACCTTATATCTGGACTTCAAGTTGGACCAAGGAGCAAAGCATTCCCAGCCTATTCC
TAAAGGGTGGACAAGCTTCATTTATACCATCTCTGGAGATGTATATATTGGGCCTGATGATGCTCAACAA
AAGATAGAACCACATCACACAGCAGTCCTTGGAGAAGGTGACGCTGTCCAGTTGGAAAATAAGGACCCCA
AAAGAAGCCATTTTGTCTTAATTGCTGGGGAGCCATTAAGAGAACCAGTTGTCCAACATGGTCCATTTGT
GATGAATACCAATGAGGAAATTTCTCAGGCCATTCTTGATTTCAGAAATGCAAAAAATGGTTTTGAGGGG
GCCAGAACCTGGAAGTCAAAGATTGGAAAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG203965 representing NM_027153
Red=Cloning site Green=Tags(s)

MASSKKVTLSVLSREQSEGVGARVRRSIGRPELKNLDPFLLFDEFKGGRPGGFPDHPHRGFETVSYLLEG
GSMAHEDFCGHVGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLRRSEKMVAPQYQELKSEEIP
KPTKDGVTVAVISGEALGIKSKVYTRTPTLYLDFKLDQGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQ
KIEPHHTAVLGEGDAVQLENKDPKRSHFVLIAGEPLREPVVQHGPFVMNTNEEISQAILDFRNAKNGFEG
ARTWKSKIGN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027153
ORF Size 870 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027153.2, NP_081429.1
RefSeq Size 1487 bp
RefSeq ORF 873 bp
Locus ID 69656
UniProt ID Q9D711
Cytogenetics X F5
Summary Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro) (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027153" in other vectors (4)
SKU Description Size Price
MC203041 Pir (untagged) - Mouse pirin (Pir), (10ug) 10 ug
$300.00
MR203965 Pir (Myc-DDK-tagged) - Mouse pirin (Pir) 10 ug
$289.00 $300.00
MR203965L3 Lenti ORF clone of Pir (Myc-DDK-tagged) - Mouse pirin (Pir) 10 ug
$600.00
MR203965L4 Lenti ORF clone of Pir (mGFP-tagged) - Mouse pirin (Pir) 10 ug
$600.00

Plasmid Map for MG203965

Download sequence for

Cloning scheme for MG203965

Circular map for MG203965

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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