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Josd3 (BC110660) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG203916
Josd3 (tGFP-tagged) - Mouse Josephin domain containing 3 (cDNA clone MGC:117821 IMAGE:3158156)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Josd3
Synonyms 5930426I11Rik, 2810003M17Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG203916 representing BC110660
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAATCAGAAGTGAATTCTGTCTGTGTGGCATCGGATAGAGCTGGAGATACTGGAGACCAAAGTG
ATGACTCCTCTGATGGCAGCTTATTTAAAACTCAGTGTGCTCCTTCACCTATACAAAAGCAAAGACACCC
TACTGTGAAACGTGTTACTTTACCTGCAAGTGTTGAAACAGATTCATCAAGTGACTCTTCTATAGAACCA
AGGCCTCTGACTTTAAAGGCTATTTTTGAAAGATTTAAAAAAAAGAAACGGAAAAAGAGGAAAAAGAGAA
AATATGAGCCAAAATTAAGACCAAGAGGAAGACCAAGAGGAAAACCATCGGGAACGAGGATCACTAGAAG
GTCGCAAATAGATGCAAAACAAATTAAAGACAAAGGAGCTGTGTTTCCATTCTTAGAATCTGAGAGTGGA
AGAAAACCTTTACCTTGGAAGAAAATTTTAACATATGAGCAAGCTGTTGCAAGAGGATTTTTTCACCACA
TTGAAAAACTCAAGTATGAACACCACCTTAAGGAATGCTTGAAGCAAATGCATGCTGGGGAAGATTTAGA
AAAGGAAGACCTTGACAGTCGGAGGCACAAATACATGGATGATGATGGTTCACTTTCTCCTATTGAAGAG
CCATTAACTGAAGATGAAGCAACAAATCCTCAGGCTGAATGTGATATCAAGTTGGTTGAAGACAGTTGCT
TCATAATAAGTTCAGAATTCTCAAGGAAGAGAAACTTGGAACAAGAGAAGATTAAGAAAGAATCTACATT
TTCTAAAAAAGCCAAAGATGCCACACACAGAGAGAAAGGGCACAGAAGAACCTTGAAAGGAAATGAACAT
GTGACTATTGAAGAAGGCTTGTCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG203916 representing BC110660
Red=Cloning site Green=Tags(s)

MAQSEVNSVCVASDRAGDTGDQSDDSSDGSLFKTQCAPSPIQKQRHPTVKRVTLPASVETDSSSDSSIEP
RPLTLKAIFERFKKKKRKKRKKRKYEPKLRPRGRPRGKPSGTRITRRSQIDAKQIKDKGAVFPFLESESG
RKPLPWKKILTYEQAVARGFFHHIEKLKYEHHLKECLKQMHAGEDLEKEDLDSRRHKYMDDDGSLSPIEE
PLTEDEATNPQAECDIKLVEDSCFIISSEFSRKRNLEQEKIKKESTFSKKAKDATHREKGHRRTLKGNEH
VTIEEGLS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC110660
ORF Size 864 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC110660, AAI10661
RefSeq Size 2254 bp
RefSeq ORF 866 bp
Locus ID 75316
Cytogenetics 9 A2
Summary Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits (By similarity).UniProtKB/Swiss-Prot Function
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"BC110660" in other vectors (4)
SKU Description Size Price
MC217912 Josd3 (untagged) - Mouse Josephin domain containing 3 (cDNA clone MGC:117821 IMAGE:3158156), (10ug) 10 ug
$330.00
MR203916 Josd3 (Myc-DDK-tagged) - Mouse Josephin domain containing 3 (cDNA clone MGC:117821 IMAGE:3158156) 10 ug
$289.00 $300.00
MR203916L3 Lenti ORF clone of Josd3 (Myc-DDK-tagged) - Mouse Josephin domain containing 3 (cDNA clone MGC:117821 IMAGE:3158156) 10 ug
$600.00
MR203916L4 Lenti ORF clone of Josd3 (mGFP-tagged) - Mouse Josephin domain containing 3 (cDNA clone MGC:117821 IMAGE:3158156) 10 ug
$600.00

Plasmid Map for MG203916

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG203916
Circular map for MG203916

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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