Clec4a2 (BC006623) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG203449
Clec4a2 (tGFP-tagged) - Mouse C-type lectin domain family 4, member a2 (cDNA clone MGC:11410 IMAGE:3968187)
  $650.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Clec4a2
Synonyms Clec4a; Clecsf6; DCIR; Dcir1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG203449 representing BC006623
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCAGAAATCACTTATGCAGAAGTGAAGTTCAAGAATGAATCCAACTCCTTGCACACCTACTCAG
AATCTCCTGCAGCTCCCAGAGAGAAACCTATCCGTGATCTAAGAAAGCCTGGTTCCCCCTCACTGCTTCT
TACATCCCTGATGCTACTTCTCCTGCTGCTGGCAATCACATTCTTAGTTGCTTTTATCATTTATTTTCAA
AAGTACTCTCAACTTCTTGAAGAAAAAAAAGCTGCAAAAAATATAATGCACAATGAATTGAACTGCACAA
AAAGTGTTTCACCCATGGAAGCACCCCCCATTGGACAAAGGGCCCTGACATTAGAGTCAATTGAGATAGA
CTTGGGTATCTTGGCACCTGAGGACAAAGTCTGGAGCTGTTGCCCAAAGGATTGGAGGCTATTTGGTTCC
CACTGCTACTTGGTTCCCACAGTTTCTTCATCAGCATCTTGGAACAAGAGTGAGGAGAACTGCTCCCGCA
TGGGTGCTCATCTAGTGGTGATCCAAAGCCAGGAAGAGCAGGATTTCATCACTGGGATCTTGGACACTCA
TGCTGCTTATTTTATAGGGTTGTGGGATACAGGCCATCGGCAATGGCAATGGGTTGATCAGACACCATAT
GAAGAAAGTATCACATTCTGGCACAATGGTGAGCCCAGCAGTGGCAATGAAAAATGTGCTACAATAATTT
ACCGTTGGAAGACTGGATGGGGCTGGAACGATATCTCTTGCAGTCTTAAACAGAAGTCAGTTTGTCAGAT
GAAGAAAATAAACTTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG203449 representing BC006623
Red=Cloning site Green=Tags(s)

MASEITYAEVKFKNESNSLHTYSESPAAPREKPIRDLRKPGSPSLLLTSLMLLLLLLAITFLVAFIIYFQ
KYSQLLEEKKAAKNIMHNELNCTKSVSPMEAPPIGQRALTLESIEIDLGILAPEDKVWSCCPKDWRLFGS
HCYLVPTVSSSASWNKSEENCSRMGAHLVVIQSQEEQDFITGILDTHAAYFIGLWDTGHRQWQWVDQTPY
EESITFWHNGEPSSGNEKCATIIYRWKTGWGWNDISCSLKQKSVCQMKKINL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC006623
ORF Size 786 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC006623, AAH06623
RefSeq Size 938 bp
RefSeq ORF 788 bp
Locus ID 26888
Cytogenetics 6 58.18 cM
Summary May be involved in regulating immune reactivity. May play a role in modulating dendritic cells (DC) differentiation and/or maturation (By similarity). May be involved in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC006623" in other vectors (1)
SKU Description Size Price
MC200710 Clec4a2 (untagged) - Mouse C-type lectin domain family 4, member a2 (cDNA clone MGC:11410 IMAGE:3968187), (10ug) 10 ug
$300.00

Plasmid Map for MG203449

Download sequence for

Cloning scheme for MG203449

Circular map for MG203449

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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