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Nt5c3l (BC015307) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG203318
Nt5c3l (tGFP-tagged) - Mouse 5'-nucleotidase, cytosolic III-like (cDNA clone MGC:19356 IMAGE:4239117)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nt5c3l
Synonyms 2610037D24Rik; AI841843; C330027I04Rik; Nt5c3l
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG203318 representing BC015307
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCTGAGCAGATTTGCTTACAATGGACAGCGATGCCCTTCTTCCCACAATATTCTGGATAACAGCA
AAATTATCAGTGAGGATTGTCGCAAAGAGCTCACCGAGCTCTTTCACCACTATTATCCCATTGAGATCGA
CCCACACCGGACCATCAAAGAGAAGCTGCCTCACATGGTGCAGTGGTGGAGCAAAGCCCACAGTCTCCTG
TGCCAGCAGAGGATCCAGAAAGTCCAGATCGCTCAGGTGGTTGGAGAGTCCACTGCGATGCTCAGGGAGG
GCTATAAGACGTTCTTCGACACACTCTACCAGAACAACATCCCCCTTTTCATCTTCTCAGCGGGCATTGG
TGACATCCTGGAAGAAATTATCCGACAGATGAAAGTGTTCCACCCCAACATCCACATTGTGTCCAACTAC
ATGGACTTCAGTGAGGATGGATTCCTGAAAGGATTCAAGGGGCAGCTCATTCACACCTACAACAAGAACA
GCTCCGTGTGTGAGAACTCCAGCTACTTCCAGCAACTTCAGAACAAGACCAACATCATCCTGCTGGGAGA
CTCCATCGGGGACCTCACCATGGCTGATGGGGTCCCCGGGGTGCAGAACATTCTCAAGATCGGCTTCCTG
AATGACAAGGTGGAGGAGCGGCGGGAGCGTTACATGGACTCCTACGACATTGTGTTGGAGAAGGACGAGA
CGCTGGACGTGGTTAATGGGTTGCTGCGGCACATCCTTTACCAGGGGGACTGCGTGGAGCTCCAGGGCTC
T

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG203318 representing BC015307
Red=Cloning site Green=Tags(s)

MTLSRFAYNGQRCPSSHNILDNSKIISEDCRKELTELFHHYYPIEIDPHRTIKEKLPHMVQWWSKAHSLL
CQQRIQKVQIAQVVGESTAMLREGYKTFFDTLYQNNIPLFIFSAGIGDILEEIIRQMKVFHPNIHIVSNY
MDFSEDGFLKGFKGQLIHTYNKNSSVCENSSYFQQLQNKTNIILLGDSIGDLTMADGVPGVQNILKIGFL
NDKVEERRERYMDSYDIVLEKDETLDVVNGLLRHILYQGDCVELQGS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC015307
ORF Size 771 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC015307, AAH15307
RefSeq Size 1390 bp
RefSeq ORF 773 bp
Locus ID 68106
Cytogenetics 11 D
Summary Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates (By similarity).UniProtKB/Swiss-Prot Function
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"BC015307" in other vectors (4)
SKU Description Size Price
MC217850 Nt5c3l (untagged) - Mouse 5'-nucleotidase, cytosolic III-like (cDNA clone MGC:19356 IMAGE:4239117), (10ug) 10 ug
$330.00
MR203318 Nt5c3l (Myc-DDK-tagged) - Mouse 5'-nucleotidase, cytosolic III-like (cDNA clone MGC:19356 IMAGE:4239117) 10 ug
$289.00 $300.00
MR203318L3 Lenti ORF clone of Nt5c3l (Myc-DDK-tagged) - Mouse 5'-nucleotidase, cytosolic III-like (cDNA clone MGC:19356 IMAGE:4239117) 10 ug
$600.00
MR203318L4 Lenti ORF clone of Nt5c3l (mGFP-tagged) - Mouse 5'-nucleotidase, cytosolic III-like (cDNA clone MGC:19356 IMAGE:4239117) 10 ug
$600.00

Plasmid Map for MG203318

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG203318
Circular map for MG203318

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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