Macrod1 (NM_134147) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG203012
Macrod1 (tGFP-tagged) - Mouse RIKEN cDNA D930010J01 gene (D930010J01Rik)
  $650.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Macrod1
Synonyms AI604841; AW743046; D930010J01Rik; Lrp16
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG203012 representing NM_134147
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCGAAGGTGGACCTGAGCACCTCCACCGACTGGAAGGAGGCCAAATCCTTTCTGAAGGGCCTGA
GTGACAAGCAGAGAGAGGAACATTACTTCTGCAAGGATTTCATTAAGCTGAAGAAGATCCCCACGTGGAA
GGAGACAGCAAAAGGGCTGGCTGTGAAGGTGGAGGACCCCAAGTATAAGAAGGACAAGCAACTGAATGAG
AAAATCTCCCTGTACCGTGGGGACATCACCAAGCTGGAGGTGGATGCCATTGTCAACGCTGCCAACAGCT
CCCTGCTTGGAGGCGGAGGGGTGGACGGCTGCATTCATCGGGCCGCGGGATCCCTGCTCACGGACGAATG
CCGCACCCTACAGAACTGCGAGACCGGCAAAGCCAAGATCACTTGCGGCTATCGGCTGCCAGCCAAGTAT
GTCATCCACACGGTGGGGCCCATCGCCGTGGGCCAACCCACTGCCAGCCAGGCGGCCGAGCTCCGCAGCT
GCTACTTGAGCAGCCTGGACCTGCTGCTGGAGCACCGGCTGCGCTCAGTGGCTTTCCCGTGCATCTCCAC
AGGCGTGTTTGGCTACCCCAATGAGGAGGCTGCAGAAGTAGTGCTGGCTTCGCTGCGGGAATGGCTGGAG
CAGCACAAGGACAAGGTGGATCGGCTCATCATCTGTGTGTTCCTGGAGAAGGACGAGGGCATTTACCGGG
AGCGCCTGCCCCATTATTTCCCAGTAGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG203012 representing NM_134147
Red=Cloning site Green=Tags(s)

MAAKVDLSTSTDWKEAKSFLKGLSDKQREEHYFCKDFIKLKKIPTWKETAKGLAVKVEDPKYKKDKQLNE
KISLYRGDITKLEVDAIVNAANSSLLGGGGVDGCIHRAAGSLLTDECRTLQNCETGKAKITCGYRLPAKY
VIHTVGPIAVGQPTASQAAELRSCYLSSLDLLLEHRLRSVAFPCISTGVFGYPNEEAAEVVLASLREWLE
QHKDKVDRLIICVFLEKDEGIYRERLPHYFPVA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_134147
ORF Size 729 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_134147.1
RefSeq Size 1229 bp
RefSeq ORF 972 bp
Locus ID 107227
UniProt ID Q922B1
Cytogenetics 19 A
Summary Removes ADP-ribose from asparatate and glutamate residues in proteins bearing a single ADP-ribose moiety. Inactive towards proteins bearing poly-ADP-ribose. Deacetylates O-acetyl-ADP ribose, a signaling molecule generated by the deacetylation of acetylated lysine residues in histones and other proteins. Plays a role in estrogen signaling. Binds to androgen receptor (AR) and amplifies the transactivation function of AR in response to androgen. May play an important role in carcinogenesis and/or progression of hormone-dependent cancers by feed-forward mechanism that activates ESR1 transactivation. Could be an ESR1 coactivator, providing a positive feedback regulatory loop for ESR1 signal transduction. Could be involved in invasive growth by down-regulating CDH1 in endometrial cancer cells. Enhances ESR1-mediated transcription activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_134147" in other vectors (4)
SKU Description Size Price
MC203738 Macrod1 (untagged) - Mouse MACRO domain containing 1 (Macrod1), (10ug) 10 ug
$450.00
MR203012 Macrod1 (Myc-DDK-tagged) - Mouse MACRO domain containing 1 (Macrod1) 10 ug
$450.00
MR203012L3 Lenti ORF clone of Macrod1 (Myc-DDK-tagged) - Mouse MACRO domain containing 1 (Macrod1) 10 ug
$750.00
MR203012L4 Lenti ORF clone of Macrod1 (mGFP-tagged) - Mouse MACRO domain containing 1 (Macrod1) 10 ug
$750.00

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