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Mbl1 (NM_010775) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202944
Mbl1 (tGFP-tagged) - Mouse mannose binding lectin (A) (Mbl1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mbl1
Synonyms MBL-A; MBP-A; S-MBP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202944 representing NM_010775
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTCTGCTTCCATTACTCCCTGTCCTTCTGTGTGTGGTGAGTGTGTCCTCATCAGGGTCACAAACCT
GTGAGGACACCCTGAAGACTTGCTCTGTGATAGCCTGTGGCAGAGATGGGAGAGATGGACCCAAAGGGGA
GAAGGGAGAACCAGGTCAAGGGCTCAGGGGCTTGCAGGGCCCTCCAGGGAAATTGGGGCCTCCAGGAAGT
GTTGGAAGCCCTGGAAGTCCAGGACCAAAAGGCCAAAAGGGGGACCATGGAGACAATAGAGCCATTGAGG
AGAAGCTGGCAAATATGGAGGCAGAGATAAGGATCCTGAAATCAAAACTGCAGCTAACCAACAAGTTGCA
TGCCTTCTCAATGGGCAAAAAGTCTGGGAAGAAGTTGTTTGTGACCAACCATGAGAAGATGCCCTTTTCC
AAAGTGAAGTCTCTGTGCACAGAGCTCCAAGGCACTGTGGCTATCCCCAGGAATGCTGAAGAGAACAAGG
CCATTCAAGAAGTGGCCACAGGCATTGCCTTCCTAGGCATCACGGACGAGGCGACTGAAGGGCAGTTCAT
GTACGTGACAGGGGGGAGGCTCACCTACAGCAACTGGAAAAAGGATGAGCCAAATAACCATGGCTCTGGG
GAAGACTGTGTCATTATATTAGATAATGGTTTGTGGAATGACATTTCCTGTCAAGCTTCCTTCAAGGCTG
TCTGCGAGTTCCCAGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202944 representing NM_010775
Red=Cloning site Green=Tags(s)

MLLLPLLPVLLCVVSVSSSGSQTCEDTLKTCSVIACGRDGRDGPKGEKGEPGQGLRGLQGPPGKLGPPGS
VGSPGSPGPKGQKGDHGDNRAIEEKLANMEAEIRILKSKLQLTNKLHAFSMGKKSGKKLFVTNHEKMPFS
KVKSLCTELQGTVAIPRNAEENKAIQEVATGIAFLGITDEATEGQFMYVTGGRLTYSNWKKDEPNNHGSG
EDCVIILDNGLWNDISCQASFKAVCEFPA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010775
ORF Size 717 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010775.2, NP_034905.1
RefSeq Size 923 bp
RefSeq ORF 720 bp
Locus ID 17194
UniProt ID P39039
Cytogenetics 14 22.36 cM
Summary Calcium-dependent lectin. Plays a role in the innate immune response by binding mannose, fucose and N-acetylglucosamine moieties on different microorganisms and mediating activation of the lectin complement pathway (By similarity). Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010775" in other vectors (4)
SKU Description Size Price
MC207326 Mbl1 (untagged) - Mouse mannose-binding lectin (protein A) 1 (Mbl1), (10ug) 10 ug
$330.00
MR202944 Mbl1 (Myc-DDK-tagged) - Mouse mannose-binding lectin (protein A) 1 (Mbl1) 10 ug
$289.00 $300.00
MR202944L3 Lenti ORF clone of Mbl1 (Myc-DDK-tagged) - Mouse mannose-binding lectin (protein A) 1 (Mbl1) 10 ug
$600.00
MR202944L4 Lenti ORF clone of Mbl1 (mGFP-tagged) - Mouse mannose-binding lectin (protein A) 1 (Mbl1) 10 ug
$600.00

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