Bpifa2 (NM_008953) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202848
Bpifa2 (tGFP-tagged) - Mouse parotid secretory protein (Psp)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bpifa2
Synonyms Bpifa2e; Psp
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202848 representing NM_008953
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCAACTTGGGAGCCTTGTTGTCTTGTGTGGCCTGCTCATTGGGAACTCAGAGTCACTTCTTGGTG
AACTTGGCAGCGCTGTGAATAATTTGAAAATTCTGAATCCTCCCTCTGAAGCTGTCCCTCAGAACCTGAA
TCTGGATGTGGAGTTGCTTCAGCAAGCCACAAGTTGGCCATTAGCCAAGAACAGCATTCTAGAAACGTTG
AACACAGCGGACCTTGGCAATTTAAAAAGCTTTACATCTTTGAATGGTTTATTGTTGAAAATCAATAATC
TCAAAGTCCTGGATTTTCAAGCTAAGCTGTCTTCCAACGGCAATGGCATTGATCTGACGGTGCCCTTGGC
TGGGGAAGCCTCCTTGGTTCTGCCTTTCATTGGCAAGACGGTCGACATTTCTGTTTCCTTGGACCTCATA
AATTCACTCTCCATTAAAACCAATGCCCAGACTGGCCTTCCTGAGGTGACCATAGGCAAATGCTCCAGTA
ATACAGATAAAATCTCCATTTCCTTGTTGGGAAGACGATTACCCATCATCAACAGTATTCTGGATGGTGT
CTCTACCCTCCTTACAAGTACATTGTCAACCGTCCTGCAAAACTTCCTATGTCCATTGCTTCAGTACGTC
CTCAGCACACTGAATCCAAGTGTTCTTCAGGGTCTCCTCTCTAATCTACTGGCAGGACAGGTACAACTTG
CCCTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202848 representing NM_008953
Red=Cloning site Green=Tags(s)

MFQLGSLVVLCGLLIGNSESLLGELGSAVNNLKILNPPSEAVPQNLNLDVELLQQATSWPLAKNSILETL
NTADLGNLKSFTSLNGLLLKINNLKVLDFQAKLSSNGNGIDLTVPLAGEASLVLPFIGKTVDISVSLDLI
NSLSIKTNAQTGLPEVTIGKCSSNTDKISISLLGRRLPIINSILDGVSTLLTSTLSTVLQNFLCPLLQYV
LSTLNPSVLQGLLSNLLAGQVQLAL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008953
ORF Size 705 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008953.2, NP_032979.1
RefSeq Size 974 bp
RefSeq ORF 708 bp
Locus ID 19194
UniProt ID P07743
Cytogenetics 2 76.15 cM
Summary Has strong antibacterial activity against P. aeruginosa.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008953" in other vectors (4)
SKU Description Size Price
MC203731 Bpifa2 (untagged) - Mouse parotid secretory protein (Psp), (10ug) 10 ug
$300.00
MR202848 Bpifa2 (Myc-DDK-tagged) - Mouse parotid secretory protein (Psp) 10 ug
$289.00 $300.00
MR202848L3 Lenti ORF clone of Bpifa2 (Myc-DDK-tagged) - Mouse parotid secretory protein (Psp) 10 ug
$600.00
MR202848L4 Lenti ORF clone of Bpifa2 (mGFP-tagged) - Mouse parotid secretory protein (Psp) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.