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Otub2 (NM_026580) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202832
Otub2 (tGFP-tagged) - Mouse OTU domain, ubiquitin aldehyde binding 2 (Otub2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
  $650.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Otub2
Synonyms 2010015L18Rik; 4930586I02Rik; AI413508; AW557219; OTB2; OTU2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202832 representing NM_026580
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGAAACATCTTTCAACCTAATATCAGAAAAATGTGACATTCTATCCATTCTTCGGGATCATCCTG
AAAACAGGATTTACCAGAGGAAAATCCAGGAACTCAGCAAAAGATTCACCTCGATCCGGAAGACCAAAGG
AGACGGAAACTGCTTCTACAGGGCCTTAGGCTATTCCTACCTGGAGTCCTTGCTGGGCAAGAGCAGAGAG
ATCCTCAAGTTCAAAGAGCGTGTGCTACAGACCCCAAATGACCTTCTGGCTGCCGGCTTTGAGGAACACA
AGTTCAGAAACTTCTTTAATGCTTTTTACAGTGTGGTTGAGCTGGTAGAGAAGGATAGCTCAGTGTCCAG
CCTGCTGAAGGTGTTCAATGACCAGAGTTCCTCGGACCGAATCGTGCAGTTCTTACGCCTCCTCACGTCG
GCCTTCATCAGGAACCGAGCTGACTTCTTCCGACATTTCATTGATGAGGAGATGGACATCAAAGACTTCT
GCACTCACGAAGTAGAGCCCATGGCCATGGAGTGTGACCACGTGCAGATCACAGCCCTGTCGCAGGCACT
CAACATTGCTCTGCAGGTAGAGTACGTCGACGAGATGGACACCGCTCTGAACCACCACGTGTTCCCCGAG
GCTGCCATCCCTTCGGTTTATCTGCTCTATAAAACATCCCACTACAACATCCTTTACGCAGCCGAGAAAC
AC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202832 representing NM_026580
Red=Cloning site Green=Tags(s)

MSETSFNLISEKCDILSILRDHPENRIYQRKIQELSKRFTSIRKTKGDGNCFYRALGYSYLESLLGKSRE
ILKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDSSVSSLLKVFNDQSSSDRIVQFLRLLTS
AFIRNRADFFRHFIDEEMDIKDFCTHEVEPMAMECDHVQITALSQALNIALQVEYVDEMDTALNHHVFPE
AAIPSVYLLYKTSHYNILYAAEKH

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026580
ORF Size 702 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026580.4, NP_080856.1
RefSeq Size 2813 bp
RefSeq ORF 705 bp
Locus ID 68149
UniProt ID Q9CQX0
Cytogenetics 12 E
Summary Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026580" in other vectors (4)
SKU Description Size Price
MC204918 Otub2 (untagged) - Mouse OTU domain, ubiquitin aldehyde binding 2 (Otub2), transcript variant 2, (10ug) 10 ug
$450.00
MR202832 Otub2 (Myc-DDK-tagged) - Mouse OTU domain, ubiquitin aldehyde binding 2 (Otub2), transcript variant 2 10 ug
$450.00
MR202832L3 Lenti ORF clone of Otub2 (Myc-DDK-tagged) - Mouse OTU domain, ubiquitin aldehyde binding 2 (Otub2), transcript variant 2 10 ug
$750.00
MR202832L4 Lenti ORF clone of Otub2 (mGFP-tagged) - Mouse OTU domain, ubiquitin aldehyde binding 2 (Otub2), transcript variant 2 10 ug
$750.00

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