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1810054G18Rik (BC013546) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202810
1810054G18Rik (tGFP-tagged) - Mouse RIKEN cDNA 1810054G18 gene (cDNA clone MGC:19331 IMAGE:4210417)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "1810054G18Rik or BC013546"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 1810054G18Rik
Synonyms 1810054G18Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202810 representing BC013546
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAAAGCCCGGAACCAGCTGGATGCCGTTCTTCAGTGTCTGCTGGAGAAGAGTCACATGGACAGGG
AACGCCTGGATGAAGAAGCTGGGAAAACCCCCTTGGATACGCACAACAAGGATTGTTCCATTGCTGCCAC
AGGCAAAAGGCCATCTGCCCGCTTCCCCCACCAGCGGAGGAAGAAGAGGAGGGAGATGGACGATGGGCTC
GCTGAGGGGGGTCCTCAGAGATCCAATACGTATGTGATCAAGCTATTTGACCGGAGTGTGGACTTGGCCC
AGTTCAGTGAGAACACACCACTGTACCCCATCTGCCGCGCCTGGATGCGCAACAGCCCCACAGTGCGAGA
GCGCGAGCGCTCACCCGGCTCACCGCTGCCTCCTCTGCCCGAGGATGGAGAGGGTTCAGAGGTCATCAAC
AGCAAAAACCGTGATGTGTACAAGCTGCCTCCACCCACAGCTCCTGGACCACTTGGAGATGCCTGTAGAT
CCAGAATTCCATCCCCACTGCAGCCTGAGACCGAGGGTACCCCCGATGATGAACCTTCCGAGCCTGAACC
TTCTCCTTCCACACTTATCTATCGCAACATGCAACGTTGGAAACGCATACGCCAGAGGTGGAAGGAGGCA
TCTCATCGGAACCAGCTTCGCTACTCAGAAAGTATGAAGATCCTACGGGAGATGTATGACCGGCAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202810 representing BC013546
Red=Cloning site Green=Tags(s)

MAKARNQLDAVLQCLLEKSHMDRERLDEEAGKTPLDTHNKDCSIAATGKRPSARFPHQRRKKRREMDDGL
AEGGPQRSNTYVIKLFDRSVDLAQFSENTPLYPICRAWMRNSPTVRERERSPGSPLPPLPEDGEGSEVIN
SKNRDVYKLPPPTAPGPLGDACRSRIPSPLQPETEGTPDDEPSEPEPSPSTLIYRNMQRWKRIRQRWKEA
SHRNQLRYSESMKILREMYDRQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC013546
ORF Size 696 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC013546, AAH13546
RefSeq Size 1002 bp
RefSeq ORF 698 bp
Locus ID 75660
Cytogenetics 7 B1
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Documents
Resources
"BC013546" in other vectors (4)
SKU Description Size Price
MC217913 1810054G18Rik (untagged) - Mouse RIKEN cDNA 1810054G18 gene (cDNA clone MGC:19331 IMAGE:4210417), (10ug) 10 ug
$330.00
MR202810 1810054G18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1810054G18 gene (cDNA clone MGC:19331 IMAGE:4210417) 10 ug
$289.00 $300.00
MR202810L3 Lenti ORF clone of 1810054G18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1810054G18 gene (cDNA clone MGC:19331 IMAGE:4210417) 10 ug
$600.00
MR202810L4 Lenti ORF clone of 1810054G18Rik (mGFP-tagged) - Mouse RIKEN cDNA 1810054G18 gene (cDNA clone MGC:19331 IMAGE:4210417) 10 ug
$600.00

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