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Psmd10 (NM_016883) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202794
Psmd10 (tGFP-tagged) - Mouse proteosome (prosome, macropain) 26S subunit, non-ATPase, 10 (Psmd10)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Psmd10 or NM_016883"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Psmd10
Synonyms AW554874
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202794 representing NM_016883
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGGTGTGTGTCTAACATAATGATCTGTAACCTGGCCTACAGTGGGAAGCTGGATGAGTTGAAGG
AGCGCATTTTGGCTGATAAATCTCTGGCTACTAGAACTGATCAGGACAGCAGAACAGCTTTGCACTGGGC
ATGCTCAGCTGGCCATACAGAAATTGTTGAATTCTTGCTGCAACTTGGAGTGCCAGTGAATGATAAAGAT
GACGCAGGTTGGTCTCCTCTTCATATTGCTGCCTCCGCTGGCCGGGATGAGATTGTAAAAGCCCTTCTGG
TGAAAGGTGCACATGTGAATGCTGTCAATCAAAACGGCTGCACTCCACTCCATTATGCAGCTTCGAAGAA
TAGGCATGAGATTGCTGTTATGTTACTAGAAGGTGGGGCTAACCCAGATGCGAAGGACCATTACGATGCT
ACAGCAATGCACCGGGCAGCAGCCAAGGGTAACTTGAAGATGGTTCACATCCTTCTGTTCTACAAAGCAT
CCACAAACATCCAAGACACTGAGGGTAACACTCCTCTACACTTAGCCTGTGATGAAGAGAGAGTGGAAGA
GGCAAAATTTCTGGTGACTCAAGGAGCAAGTATTTACATTGAGAATAAAGAAGAAAAGACACCCCTGCAA
GTGGCCAAAGGGGGCCTGGGTTTAATACTCAAGAGACTAGCAGAAAGTGAAGAGGCTTCTATG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202794 representing NM_016883
Red=Cloning site Green=Tags(s)

MEGCVSNIMICNLAYSGKLDELKERILADKSLATRTDQDSRTALHWACSAGHTEIVEFLLQLGVPVNDKD
DAGWSPLHIAASAGRDEIVKALLVKGAHVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYDA
TAMHRAAAKGNLKMVHILLFYKASTNIQDTEGNTPLHLACDEERVEEAKFLVTQGASIYIENKEEKTPLQ
VAKGGLGLILKRLAESEEASM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016883
ORF Size 693 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016883.4
RefSeq Size 1356 bp
RefSeq ORF 696 bp
Locus ID 53380
UniProt ID Q9Z2X2
Cytogenetics X F1
Summary Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module (By similarity). Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016883" in other vectors (4)
SKU Description Size Price
MC201453 Psmd10 (untagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (Psmd10), transcript variant 1, (10ug) 10 ug
$300.00
MR202794 Psmd10 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (Psmd10), transcript variant 1 10 ug
$289.00 $300.00
MR202794L3 Lenti ORF clone of Psmd10 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (Psmd10), transcript variant 1 10 ug
$600.00
MR202794L4 Lenti ORF clone of Psmd10 (mGFP-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (Psmd10), transcript variant 1 10 ug
$600.00

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