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Lypla2 (NM_011942) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202786
Lypla2 (tGFP-tagged) - Mouse lysophospholipase 2 (Lypla2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lypla2
Synonyms LysoII
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202786 representing NM_011942
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTGGTAACACCATGTCTGTGCCCCTGCTCACCGACGCAGCCACCGTGTCTGGAGCTGAGCGGGAAA
CGGCCGCGGTTATTTTTTTACATGGACTTGGAGACACAGGGCACAGCTGGGCTGACGCCCTCTCCACCAT
CCGGCTTCCTCATGTCAAGTACATCTGTCCCCATGCGCCCAGGATCCCTGTGACACTCAACATGAAGATG
GTGATGCCCTCCTGGTTTGACCTGATGGGGCTGAGTCCGGATGCCCCAGAGGATGAAGCTGGCATCAAGA
AGGCCGCAGAGAACATCAAGGCTTTGATTGAACATGAGATGAAGAACGGGATTCCTGCCAACCGGATCGT
CCTGGGTGGCTTCTCGCAGGGTGGGGCCCTGTCCCTCTATACAGCACTTACCTGCCCCCACCCTCTGGCT
GGCATTGTGGCATTGAGCTGCTGGCTGCCTCTGCACCGGAACTTCCCCCAAGCAGCCAATGGCAGTGCCA
AGGACCTGGCCATCCTTCAGTGCCACGGGGAGCTGGACCCCATGGTACCTGTTCGGTTTGGAGCCCTGAC
AGCTGAGAAGCTCCGGACTGTTGTCACACCTGCCAGGGTCCAGTTTAAGACATACCCAGGTGTCATGCAC
AGCTCCTGTCCTCAGGAGATGGCAGCTGTAAAGGAATTTCTGGAGAAACTTCTGCCTCCGGTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202786 representing NM_011942
Red=Cloning site Green=Tags(s)

MCGNTMSVPLLTDAATVSGAERETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKM
VMPSWFDLMGLSPDAPEDEAGIKKAAENIKALIEHEMKNGIPANRIVLGGFSQGGALSLYTALTCPHPLA
GIVALSCWLPLHRNFPQAANGSAKDLAILQCHGELDPMVPVRFGALTAEKLRTVVTPARVQFKTYPGVMH
SSCPQEMAAVKEFLEKLLPPV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011942
ORF Size 693 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011942.1, NP_036072.1
RefSeq Size 1593 bp
RefSeq ORF 696 bp
Locus ID 26394
UniProt ID Q9WTL7
Cytogenetics 4 D3
Summary Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins, GAP43, ZDHHC6 or HRAS. Deacylates GAP43. Mediates depalmitoylation of ZDHHC6 (By similarity). Has lysophospholipase activity (PubMed:10064901).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011942" in other vectors (4)
SKU Description Size Price
MC202502 Lypla2 (untagged) - Mouse lysophospholipase 2 (Lypla2), (10ug) 10 ug
$300.00
MR202786 Lypla2 (Myc-DDK-tagged) - Mouse lysophospholipase 2 (Lypla2) 10 ug
$289.00 $300.00
MR202786L3 Lenti ORF clone of Lypla2 (Myc-DDK-tagged) - Mouse lysophospholipase 2 (Lypla2) 10 ug
$600.00
MR202786L4 Lenti ORF clone of Lypla2 (mGFP-tagged) - Mouse lysophospholipase 2 (Lypla2) 10 ug
$600.00

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