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Thyn1 (NM_144543) Mouse Tagged ORF Clone

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SKU
MG202670
Thyn1 (tGFP-tagged) - Mouse thymocyte nuclear protein 1 (Thyn1)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Thyn1
Synonyms D730042P09Rik; HSPC144; MDS012; MY105; Thy28
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202670 representing NM_144543
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGAGACCCCGCAAGAGGCAGACTGGGACTGCTGGTCCAGACAGGAAGAAGCTATCCGGGAAACGTA
CTAAAACTGAAAACTCAGAAAGTACATCAGTCAAGCTAGAGAACTCCAGCCTTGAGATGACGACAACCTT
TAAAAATGGTGGCAAGAATTTAAGCAACTACTGGCTGATGAAGTCAGAGCCAGAAAGCCGGCTAGAGAAA
GGTATAGACATGAAGTTCAGCATCGAGGATCTCAAAGCACAGCCCAAGCAGACAGCTTGCTGGGATGGTG
TTCGCAACTATCAGGCTCGGAACTTCCTCAGGGCCATGAAATTGGAGGATGAAGCCTTCTTCTACCATAG
CAACTGCAAACAGCCTGGCATTGTTGGACTTATGAAGATTGTAAAGGAGGCTTACCCAGACCATACACAG
TTTGAGAAAAGTAACCCCCATTACGATCCATCCAGCAAAGAGGACGACCCTAAATGGTCGATGGTGGATG
TACAGTTTGTTCGAATGATGAAGCGCTTCATCCCTCTGGAGGAGCTTAAAACCTATCACCAAGCCCACAA
AGCTACTGGCGGCCCCTTAAAAAGCATGACTCTCTTCACCCGCCAGAGACTTTCCGTTCAGCCTCTGACT
CAAGAAGAGTTTGATTTTATTTTGAGCCTGGAGGAAACAGAACCAAGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202670 representing NM_144543
Red=Cloning site Green=Tags(s)

MPRPRKRQTGTAGPDRKKLSGKRTKTENSESTSVKLENSSLEMTTTFKNGGKNLSNYWLMKSEPESRLEK
GIDMKFSIEDLKAQPKQTACWDGVRNYQARNFLRAMKLEDEAFFYHSNCKQPGIVGLMKIVKEAYPDHTQ
FEKSNPHYDPSSKEDDPKWSMVDVQFVRMMKRFIPLEELKTYHQAHKATGGPLKSMTLFTRQRLSVQPLT
QEEFDFILSLEETEPS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144543
ORF Size 678 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144543.1, NP_653126.1
RefSeq Size 930 bp
RefSeq ORF 681 bp
Locus ID 77862
UniProt ID Q91YJ3
Cytogenetics 9 A4
Summary Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_144543" in other vectors (3)
SKU Description Size Price
MR202670 Thyn1 (Myc-DDK-tagged) - Mouse thymocyte nuclear protein 1 (Thyn1) 10 ug
$289.00 $300.00
MR202670L3 Lenti ORF clone of Thyn1 (Myc-DDK-tagged) - Mouse thymocyte nuclear protein 1 (Thyn1) 10 ug
$600.00
MR202670L4 Lenti ORF clone of Thyn1 (mGFP-tagged) - Mouse thymocyte nuclear protein 1 (Thyn1) 10 ug
$600.00

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