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Rnf186 (NM_025786) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202649
Rnf186 (tGFP-tagged) - Mouse ring finger protein 186 (Rnf186)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rnf186
Synonyms 9130020G10Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202649 representing NM_025786
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTGCACTGAGGCCCCACAGCCTATCCCAGCGGGTACCACCACCACCAGCACCATCATTGCCTTGG
GGCCCACCGGGCGGCTCAGCATCTCTGTGGAGGGTGACCTGGAATGCTTGGTGTGCCGAGAGCCCTACAA
CTGTGCTCGGTCCCCCAAGCTGCTTAGCTGTCAGCATACCTTCTGTGCCGTATGCCTGAAGCTTCTGCTA
TATGTGCAGGAAGACACCTGGTCCATCCCCTGTCCGCTGTGCCGAAAGGTCACTGCTGTCCCGGGAGGCC
TCATCTGCAGCTTGCGAGACCAGGAGGCGATGGTGGGGCGTCTGGCCCTGCCATGCCCAGAGGTGCGCCT
ATGTCCTCAGAGGCTAGTGGGTTCTGCCGCTTCAGCAACTCGGCCAGCCAACTGGACAGGAGAAGAAGAA
CAGGACACTGTAAGTGTCAACCGTGTAGCTGCCCGGCGCCTGGCTGTACACTTGCTCTTGTTGGCTCTTG
TCATTGTCCTTATCCTGCCTTTCATCTACCCGGGTGTCATCCGGTGGGTGTTGGCCTTTGTCATTGCTCT
GGCTCTCCTGATGTCCACCCTATTCTGCTGTCACCCCCAGAGCCAGAACAGCAACTGGCTTTGCCCCAGG
ACTCTGTTCTGCAGAGAGCAAAAGCAAACCCAGATCACTTCTATTGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202649 representing NM_025786
Red=Cloning site Green=Tags(s)

MSCTEAPQPIPAGTTTTSTIIALGPTGRLSISVEGDLECLVCREPYNCARSPKLLSCQHTFCAVCLKLLL
YVQEDTWSIPCPLCRKVTAVPGGLICSLRDQEAMVGRLALPCPEVRLCPQRLVGSAASATRPANWTGEEE
QDTVSVNRVAARRLAVHLLLLALVIVLILPFIYPGVIRWVLAFVIALALLMSTLFCCHPQSQNSNWLCPR
TLFCREQKQTQITSIA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025786
ORF Size 678 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025786.2, NP_080062.3
RefSeq Size 1244 bp
RefSeq ORF 843 bp
Locus ID 66825
UniProt ID Q9D241
Cytogenetics 4 D3
Summary E3 ubiquitin protein ligase that is part of an apoptotic signaling pathway activated by endoplasmic reticulum stress. In that process, stimulates the expression of proteins specific of the unfolded protein response (UPR), ubiquitinates BNIP1 and regulates its localization to the mitochondrion and induces calcium release from the endoplasmic reticulum that ultimately leads to cell apoptosis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025786" in other vectors (3)
SKU Description Size Price
MR202649 Rnf186 (Myc-DDK-tagged) - Mouse ring finger protein 186 (Rnf186) 10 ug
$289.00 $300.00
MR202649L3 Lenti ORF clone of Rnf186 (Myc-DDK-tagged) - Mouse ring finger protein 186 (Rnf186) 10 ug
$600.00
MR202649L4 Lenti ORF clone of Rnf186 (mGFP-tagged) - Mouse ring finger protein 186 (Rnf186) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.