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Atpbd4 (BC022995) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202634
Atpbd4 (tGFP-tagged) - Mouse ATP binding domain 4 (cDNA clone MGC:35721 IMAGE:5363234)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Atpbd4 or BC022995"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atpbd4
Synonyms 5730421E18Rik; Atpbd4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202634 representing BC022995
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGTCGCGGCCCTGATCAGTGGTGGGAAGGACAGCTGTTACAACATGATGCAGTGCATTGCTGAGG
GGCATCAAATTGTTGCATTAGCAAATCTAAGACCAGATGAAAACCAAGTGGAGTCAGATGAACTGGATAG
CTATATGTATCAGACAGTGGGTCACCATGCCATTGACTTGTATGCTGAAGCAATGGCGCTGCCCCTGTAT
CGCAGAGCCATCAGAGGAAGGAGCTTGGAGACAGGAAGAGTTTATACGCAATGTGAAGGTGACGAGGTTG
AAGATCTCTATGAACTGTTGAAACTTGTTAAGGAAAAAGAAGAAATCGAAGGGGTATCAGTAGGTGCTAT
ACTCTCTGACTATCAACGTGGACGAGTAGAAAATGTATGTAAACGACTCAATCTCCAGCCTTTAGCTTAT
CTTTGGCAAAGAAACCAGGAAGATTTGCTCCGAGAGATGATAGCTTCTAATATCAAGGCCATTATCATCA
AAGTAGCAGCTTTGGGCTTAGATCCTGATAAGCATCTTGGGAAAACCCTGGTTGAAATGGAGCCTTATCT
TTTAGAGGGACTCTTCAGAAGCAGTCATGCACTCAGCGGATGCATTCGCACCTGTGGCTTATCTGCGGCT
CTCCCGGCTGCACTTGGAAGAGAAAGTGTCGTCAGTACCTGCGGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202634 representing BC022995
Red=Cloning site Green=Tags(s)

MRVAALISGGKDSCYNMMQCIAEGHQIVALANLRPDENQVESDELDSYMYQTVGHHAIDLYAEAMALPLY
RRAIRGRSLETGRVYTQCEGDEVEDLYELLKLVKEKEEIEGVSVGAILSDYQRGRVENVCKRLNLQPLAY
LWQRNQEDLLREMIASNIKAIIIKVAALGLDPDKHLGKTLVEMEPYLLEGLFRSSHALSGCIRTCGLSAA
LPAALGRESVVSTCG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC022995
ORF Size 675 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC022995, AAH22995
RefSeq Size 2693 bp
RefSeq ORF 677 bp
Locus ID 66632
Cytogenetics 2 E4
Summary Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor 2 (EEF2) to diphthamide (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC022995" in other vectors (4)
SKU Description Size Price
MC206904 Atpbd4 (untagged) - Mouse ATP binding domain 4 (cDNA clone MGC:35721 IMAGE:5363234), (10ug) 10 ug
$330.00
MR202634 Atpbd4 (Myc-DDK-tagged) - Mouse ATP binding domain 4 (cDNA clone MGC:35721 IMAGE:5363234) 10 ug
$289.00 $300.00
MR202634L3 Lenti ORF clone of Atpbd4 (Myc-DDK-tagged) - Mouse ATP binding domain 4 (cDNA clone MGC:35721 IMAGE:5363234) 10 ug
$600.00
MR202634L4 Lenti ORF clone of Atpbd4 (mGFP-tagged) - Mouse ATP binding domain 4 (cDNA clone MGC:35721 IMAGE:5363234) 10 ug
$600.00

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