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Chkb (BC068195) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202606
Chkb (tGFP-tagged) - Mouse choline kinase beta (cDNA clone MGC:70277 IMAGE:4164116)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Chkb or BC068195"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Chkb
Synonyms CK/EK, CK/EK-beta
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202606 representing BC068195
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCGTTTCCATGGTATGGAGATGCCCTTCACTAAGGAGCCCCGATGGTTGTTTGGGACCATGGAGC
GGTACCTGAAGCAGATCCAGGACTTGCCTTCCACCAGCCTTCCCCAGATGAACCTGGTAGAGATGTACAG
CCTCAAGGACGAGATGAACAGCCTCAGGAAGTTACTAGACGATACCCCGTCACCAGTGGTCTTCTGCCAC
AATGACATCCAGGAAGGAAACATCTTGTTGCTTTCAGAGCCAGACAGTGATGATAACCTCATGTTGGTTG
ATTTTGAGTACAGTAGTTATAACTACAGGGGCTTTGACATTGGGAATCATTTTTGTGAGTGGGTTTATGA
TTATACTTATGAAGAATGGCCTTTCTACAAAGCAAGACCCACGGACTACCCCACTAGAGAACAGCAGCTC
CATTTTATTCGCCATTATCTGGCAGAGGTTCAGAAGGGTGAGATCCTCTCCGAAGAGGAACAGAAGAAAC
GGGAAGAAGAATTGCTGCTAGAGATCAGTCGGTACTCCCTGGCATCTCACTTTTTCTGGGGTCTGTGGTC
CACCCTGCAGGCTTCGATGTCCACTATAGAGTTCGGCTACTTGGAGTATGCCCAATCTCGGTTCCAGTTC
TACTTCCAGCAGAAGGGCCAGCTGACGAGTTCCCCATCATCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202606 representing BC068195
Red=Cloning site Green=Tags(s)

MARFHGMEMPFTKEPRWLFGTMERYLKQIQDLPSTSLPQMNLVEMYSLKDEMNSLRKLLDDTPSPVVFCH
NDIQEGNILLLSEPDSDDNLMLVDFEYSSYNYRGFDIGNHFCEWVYDYTYEEWPFYKARPTDYPTREQQL
HFIRHYLAEVQKGEILSEEEQKKREEELLLEISRYSLASHFFWGLWSTLQASMSTIEFGYLEYAQSRFQF
YFQQKGQLTSSPSS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC068195
ORF Size 672 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC068195, AAH68195
RefSeq Size 1278 bp
RefSeq ORF 674 bp
Locus ID 12651
Cytogenetics 15 E3
Summary Has a key role in phospholipid metabolism, and catalyzes the first step of phosphatidylethanolamine and phosphatidylcholine biosynthesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC068195" in other vectors (4)
SKU Description Size Price
MC206825 Chkb (untagged) - Mouse choline kinase beta (cDNA clone MGC:70277 IMAGE:4164116), (10ug) 10 ug
$330.00
MR202606 Chkb (Myc-DDK-tagged) - Mouse choline kinase beta (cDNA clone MGC:70277 IMAGE:4164116) 10 ug
$289.00 $300.00
MR202606L3 Lenti ORF clone of Chkb (Myc-DDK-tagged) - Mouse choline kinase beta (cDNA clone MGC:70277 IMAGE:4164116) 10 ug
$600.00
MR202606L4 Lenti ORF clone of Chkb (mGFP-tagged) - Mouse choline kinase beta (cDNA clone MGC:70277 IMAGE:4164116) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.