Cbln2 (NM_172633) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202603
Cbln2 (tGFP-tagged) - Mouse cerebellin 2 precursor protein (Cbln2)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cbln2
Synonyms 6330593N19Rik; A730004O05
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202603 representing NM_172633
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCGCGCCTGGCCAGGGCCCCAGAGGGCCGCTGCTGAGCATGCCCGGGCGCCGGGGGGCGCTGCGTG
AGCCAGCCGACTTTGGCTCCAGCCTGGGGGCGGTGCTGGCCCTGCTGTTGCTGCTGCTGCCCGCCTGCTG
CCCCGTAAGGGCTCAGAACGACACGGAGCCCATCGTGCTAGAGGGCAAGTGCCTGGTAGTGTGCGATTCC
AGCCCATCGGGGGATGGCGCCGTCACTTCTTCCCTGGGCATTTCTGTGCGCTCAGGCAGTGCCAAGGTGG
CCTTCTCCGCTACTCGGAGCACCAACCACGAGCCGTCAGAGATGAGCAACCGTACCATGACCATCTACTT
CGACCAGGTCTTAGTAAACATTGGCAACCACTTTGACCTTGCCTCCAGTATATTTGTAGCACCAAGAAAG
GGAATTTATAGCTTCAGCTTCCACGTGGTCAAAGTGTACAACAGACAAACTATCCAGGTCAGCTTAATGC
AGAATGGCTACCCGGTGATCTCTGCATTTGCCGGAGACCAGGATGTTACCAGGGAAGCAGCCAGCAATGG
TGTTCTGCTGCTCATGGAAAGAGAAGACAAAGTTCATCTCAAACTAGAGAGAGGCAACCTCATGGGAGGC
TGGAAATACTCCACATTCTCGGGCTTCTTGGTTTTTCCTCTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202603 representing NM_172633
Red=Cloning site Green=Tags(s)

MPAPGQGPRGPLLSMPGRRGALREPADFGSSLGAVLALLLLLLPACCPVRAQNDTEPIVLEGKCLVVCDS
SPSGDGAVTSSLGISVRSGSAKVAFSATRSTNHEPSEMSNRTMTIYFDQVLVNIGNHFDLASSIFVAPRK
GIYSFSFHVVKVYNRQTIQVSLMQNGYPVISAFAGDQDVTREAASNGVLLLMEREDKVHLKLERGNLMGG
WKYSTFSGFLVFPL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172633
ORF Size 672 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172633.4
RefSeq Size 2202 bp
RefSeq ORF 675 bp
Locus ID 12405
UniProt ID Q8BGU2
Cytogenetics 18 58.63 cM
Summary The protein encoded by this gene belongs to a family of secreted neuronal glycoproteins. The transcript is broadly expressed in the embryonic and adult brain with higher levels in some regions including the olfactory bulb, thalamus, and cerebral cortex. The protein can bind to presynaptic neurexins and induce synaptogenesis in cultured neurons. Null mutant mice are viable, fertile and do not display obvious neuroanatomical defects. Alternative splicing results in multiple transcript variants. provided by RefSeq, Oct 2014
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Documents
Resources
"NM_172633" in other vectors (4)
SKU Description Size Price
MC202566 Cbln2 (untagged) - Mouse cerebellin 2 precursor protein (Cbln2), (10ug) 10 ug
$300.00
MR202603 Cbln2 (Myc-DDK-tagged) - Mouse cerebellin 2 precursor protein (Cbln2) 10 ug
$289.00 $300.00
MR202603L3 Lenti ORF clone of Cbln2 (Myc-DDK-tagged) - Mouse cerebellin 2 precursor protein (Cbln2) 10 ug
$600.00
MR202603L4 Lenti ORF clone of Cbln2 (mGFP-tagged) - Mouse cerebellin 2 precursor protein (Cbln2) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.