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Chac1 (NM_026929) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202572
Chac1 (tGFP-tagged) - Mouse ChaC, cation transport regulator-like 1 (E. coli) (Chac1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Chac1 or NM_026929"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Chac1
Synonyms 1810008K03Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202572 representing NM_026929
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCAAGAGTCCGCATCCCAGAGCACTCCGCCTCCTTCACTGTCCCCTGCACCATCATCCGCGCAGC
CTTTCTGGGGGGATGGCGACCCCCAAGCCCTGTGGATTTTCGGGTACGGCTCCCTAGTGTGGAAGCCGGA
CTTTGCCTATAGTGACAGCCGTGTGGGCTTCGTTCGTGGCTATAGCCGACGGTTCTGGCAGGGAGACACC
TTCCATAGGGGCAGCGACAAGATGCCTGGCCGAGTGGTGACCCTCCTTGAAGACCATGAGGGCTGCACTT
GGGGTGTGGCATACCAAGTTCGAGGGGAGCAGGTGAACGAGGCCCTGAAGTACCTGAATGTGAGGGAAGC
CGTGCTTGGTGGCTATGACACTAAGGAAGTCACCTTTTATCCTCAAGACACCCCTGACCAACCCCTCACA
GCACTGGCCTATGTGGCCACCCCACAGAACCCTGGCTACCTGGGCCCTGCTCCTGAAGAGGTCATTGCCA
CACAGATCCTTGCTTGCCGAGGCTTCTCTGGTCACAACCTTGAGTACTTATTGCGTTTGGCAGACTTCAT
GCAGCTCTGTGGGCCTCAGGCACAAGATGAGCACCTGGAAGCCATTGTGGACGCCGTAGGAACCCTGCTA
CCCTGCTCTTACCTCCCTGAGCAGCCTCTGGCACTGACC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202572 representing NM_026929
Red=Cloning site Green=Tags(s)

MKQESASQSTPPPSLSPAPSSAQPFWGDGDPQALWIFGYGSLVWKPDFAYSDSRVGFVRGYSRRFWQGDT
FHRGSDKMPGRVVTLLEDHEGCTWGVAYQVRGEQVNEALKYLNVREAVLGGYDTKEVTFYPQDTPDQPLT
ALAYVATPQNPGYLGPAPEEVIATQILACRGFSGHNLEYLLRLADFMQLCGPQAQDEHLEAIVDAVGTLL
PCSYLPEQPLALT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026929
ORF Size 669 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026929.2, NP_081205.1
RefSeq Size 1613 bp
RefSeq ORF 672 bp
Locus ID 69065
UniProt ID Q8R3J5
Cytogenetics 2 E5
Summary Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (By similarity). Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed:22445366).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026929" in other vectors (6)
SKU Description Size Price
MC202240 Chac1 (untagged) - Mouse ChaC, cation transport regulator-like 1 (E. coli) (Chac1), (10ug) 10 ug
$300.00
MR202572 Chac1 (Myc-DDK-tagged) - Mouse ChaC, cation transport regulator-like 1 (E. coli) (Chac1) 10 ug
$289.00 $300.00
MR202572L1 Lenti ORF clone of Chac1 (Myc-DDK-tagged) - Mouse ChaC, cation transport regulator-like 1 (E. coli) (Chac1) 10 ug
$600.00
MR202572L2 Lenti ORF clone of Chac1 (mGFP-tagged) - Mouse ChaC, cation transport regulator-like 1 (E. coli) (Chac1) 10 ug
$600.00
MR202572L3 Lenti ORF clone of Chac1 (Myc-DDK-tagged) - Mouse ChaC, cation transport regulator-like 1 (E. coli) (Chac1) 10 ug
$600.00
MR202572L4 Lenti ORF clone of Chac1 (mGFP-tagged) - Mouse ChaC, cation transport regulator-like 1 (E. coli) (Chac1) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.