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Mmachc (NM_025962) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202551
Mmachc (tGFP-tagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Mmachc or NM_025962"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mmachc
Synonyms 1810037K07Rik; CblC
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202551 representing NM_025962
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTGACAGAGCCCTCAAACCCTTCTTAAAGAGCTGCCACTTCCAAACACTGAGAGACCCGGTGGATC
AATGTGTGTCCTACCACCTGAGGAGTGTTACAGAGAAGTTTCCAGAAGTGCATATGGAAGTCATTGCTGA
CTATGAGGTACACCCCAATCGGCGACCTAAGATTCTCGCCCAGACAGCAGCCCATGTGGCAGGTGCTGCT
TATTACTACCAACGACAAGATGTGGATGCAGACCCATGGGGGACCCAGCACATAGCAGGTGTGTGCATAC
ACCCCCGATTTGGGGGCTGGTTTGCCATCCGAGGGGTTATGTTGCTGCCAGGGATTGAAGTGCCAAATTT
GCCACCCAGAAAGCCCCCTGACTGTGTGCCTACAAGAGCTGGCCGCATCACTCTGCTTGAAGGTTTCAAT
TTCCATTGGCGGGACTGGACTTACCGTGATGCTGTGACTCCTGAAGAACGGTACTCCGAAGAACAGAAGA
TCTACTTTTCCACCCCACCTGCCCAACGCTTGGCCCTATTAGGCTTAGCCCAACCCTCAGAACACCCTAG
CACTACATCTGAGCTTCCTCTTTCCTTGCTTACTAAACCTCAGAATTCCAGGAGAGCTCGAAGCTGGCTG
AGTCCAAGTGTCTCACCACCTGTATCCCCAGGCCCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202551 representing NM_025962
Red=Cloning site Green=Tags(s)

MFDRALKPFLKSCHFQTLRDPVDQCVSYHLRSVTEKFPEVHMEVIADYEVHPNRRPKILAQTAAHVAGAA
YYYQRQDVDADPWGTQHIAGVCIHPRFGGWFAIRGVMLLPGIEVPNLPPRKPPDCVPTRAGRITLLEGFN
FHWRDWTYRDAVTPEERYSEEQKIYFSTPPAQRLALLGLAQPSEHPSTTSELPLSLLTKPQNSRRARSWL
SPSVSPPVSPGP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025962
ORF Size 666 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025962.2
RefSeq Size 1863 bp
RefSeq ORF 840 bp
Locus ID 67096
UniProt ID Q9CZD0
Cytogenetics 4 D1
Summary Catalyzes the reductive dealkylation of cyanocobalamin to cob(II)alamin, using FAD or FMN as cofactor and NADPH as cosubstrate. Can also catalyze the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. Under anaerobic conditions cob(I)alamin is the first product; it is highly reactive and is converted to aquocob(II)alamin in the presence of oxygen. Binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025962" in other vectors (4)
SKU Description Size Price
MC202568 Mmachc (untagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc), (10ug) 10 ug
$300.00
MR202551 Mmachc (Myc-DDK-tagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc) 10 ug
$289.00 $300.00
MR202551L3 Lenti ORF clone of Mmachc (Myc-DDK-tagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc) 10 ug
$600.00
MR202551L4 Lenti ORF clone of Mmachc (mGFP-tagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.