Rab28 (NM_027295) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202539
Rab28 (tGFP-tagged) - Mouse RAB28, member RAS oncogene family (Rab28)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rab28
Synonyms 2700023P08Rik; AW496496
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202539 representing NM_027295
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGACTCTGAGGAGGAGAGCCAGGACCGGCAACTGAAAATCGTCGTGCTGGGGGACGGCACCTCGG
GGAAGACCTCCTTAGCTACGTGTTTTGCTCAAGAGACATTTGGGAAGCAGTACAAGCAGACGATAGGACT
GGATTTCTTTTTGAGAAGGATTACCTTACCAGGAAATTTGAATGTTACTCTTCAAGTTTGGGATATAGGA
GGGCAGACAATAGGTGGCAAGATGTTGGATAAATACATCTATGGAGCACAGGGAATCCTCTTGGTATATG
ATATTACAAACTACCAAAGCTTTGAGAATTTGGAAGATTGGTATTCTGTGGTGAAGACAGTAAGTGAAGA
GTCAGAAACTCAGCCCCTGGTTGCTTTAGTGGGCAATAAAATTGACTTGGAGCATATGCGAACAGTAAAA
GCTGATAAGCACTTACGATTTTGCCAGGAAAATGGTTTTAGTAGCCACTTTGTTTCAGCCAAGACAGGAG
ACTCTGTCTTCCTGTGTTTTCAGAAAGTTGCAGCAGAAATCCTGGGAATCAAATTAAACAAAGCAGAAAT
AGAACAGTCACAGAGAGTGGTGAAGGCAGATATTGTAAACTACAACCAGGAGCCCTTGTCAAGAACTGTT
AACCCTCCTCGGAGCTCCATGTGTGCAGTGCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202539 representing NM_027295
Red=Cloning site Green=Tags(s)

MSDSEEESQDRQLKIVVLGDGTSGKTSLATCFAQETFGKQYKQTIGLDFFLRRITLPGNLNVTLQVWDIG
GQTIGGKMLDKYIYGAQGILLVYDITNYQSFENLEDWYSVVKTVSEESETQPLVALVGNKIDLEHMRTVK
ADKHLRFCQENGFSSHFVSAKTGDSVFLCFQKVAAEILGIKLNKAEIEQSQRVVKADIVNYNQEPLSRTV
NPPRSSMCAVQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027295
ORF Size 663 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027295.3
RefSeq Size 1636 bp
RefSeq ORF 666 bp
Locus ID 100972
UniProt ID Q99KL7
Cytogenetics 5 B3
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Documents
Resources
"NM_027295" in other vectors (4)
SKU Description Size Price
MC200213 Rab28 (untagged) - Mouse RAB28, member RAS oncogene family (Rab28), (10ug) 10 ug
$300.00
MR202539 Rab28 (Myc-DDK-tagged) - Mouse RAB28, member RAS oncogene family (Rab28) 10 ug
$289.00 $300.00
MR202539L3 Lenti ORF clone of Rab28 (Myc-DDK-tagged) - Mouse RAB28, member RAS oncogene family (Rab28) 10 ug
$600.00
MR202539L4 Lenti ORF clone of Rab28 (mGFP-tagged) - Mouse RAB28, member RAS oncogene family (Rab28) 10 ug
$600.00

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