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Ier2 (NM_010499) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202538
Ier2 (tGFP-tagged) - Mouse immediate early response 2 (Ier2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ier2
Synonyms AI317238; Ch1; Pip92
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202538 representing NM_010499
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGTACAGAAAGAAGCGCAGCGCATCATGACTCTGTCGGTATGGAAGATGTACCACTCTCGCATGC
AGCGAGGTGGCTTGCGACTCCACCGGAGTCTGCAGCTGTCCCTCGTTATGCGCAGCGCTCGAGAGGTCTA
CCTCTCAGCCAAGGTAGAAGCCCACCAGCCCGAGTTCCCGCCATCCCGCAGGGCTCTTGACCCTCGCCTG
CACCCGCCGCGGGAAGCCGAAGTTGCAGTGGAAGTAGCGTCCCCCGAAGCCGTGCAGCCTCCGGAGCCCA
TGGATACGCAAGAGGAAGTGCTGCGAGTCCAGGAGACCCCTGCGCTCTGTGACCCGCCCCCCGCTAGAGT
CAGCCGCAAGCGCCGGAGCAGCAGCGATTTGAGCGACGGTAGTGATGCCGGACTGGTACCAAGCAAGAAG
GCCCGTCTAGAAGAGGTGGAGGGGGAGGCGACGTCGGAGGTTCCCGATCGCCTGCAGCTTCCTCCGGCAC
AAAGCGAAGGTGCCTTCCCTAACCTCGCCCGCGTCCTCCAAAGGCGCTTCTCCAGTCTCCTGAACTGTGG
ACCCGCCGTGCCCCCGCCGACGCCCCCCACGTGCGAGGCCAAGCCAGCCTGCCGCCCGGCCGACAATATG
CTCAACGTGCTGGTGCGAGCTGTGGTGGCCTTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202538 representing NM_010499
Red=Cloning site Green=Tags(s)

MEVQKEAQRIMTLSVWKMYHSRMQRGGLRLHRSLQLSLVMRSAREVYLSAKVEAHQPEFPPSRRALDPRL
HPPREAEVAVEVASPEAVQPPEPMDTQEEVLRVQETPALCDPPPARVSRKRRSSSDLSDGSDAGLVPSKK
ARLEEVEGEATSEVPDRLQLPPAQSEGAFPNLARVLQRRFSSLLNCGPAVPPPTPPTCEAKPACRPADNM
LNVLVRAVVAF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010499
ORF Size 663 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010499.4, NP_034629.3
RefSeq Size 3302 bp
RefSeq ORF 666 bp
Locus ID 15936
UniProt ID P17950
Cytogenetics 8 41.02 cM
Summary DNA-binding protein that seems to act as a transcription factor (By similarity). Involved in the regulation of neuronal differentiation, acts upon JNK-signaling pathway activation and plays a role in neurite outgrowth in hippocampal cells (By similarity). May mediate with FIBP FGF-signaling in the establishment of laterality in the embryo (By similarity). Promotes cell motility, seems to stimulate tumor metastasis (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010499" in other vectors (4)
SKU Description Size Price
MC208026 Ier2 (untagged) - Mouse immediate early response 2 (Ier2), (10ug) 10 ug
$300.00
MR202538 Ier2 (Myc-DDK-tagged) - Mouse immediate early response 2 (Ier2) 10 ug
$289.00 $300.00
MR202538L3 Lenti ORF clone of Ier2 (Myc-DDK-tagged) - Mouse immediate early response 2 (Ier2) 10 ug
$600.00
MR202538L4 Lenti ORF clone of Ier2 (mGFP-tagged) - Mouse immediate early response 2 (Ier2) 10 ug
$600.00

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