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Tspan2 (NM_027533) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202532
Tspan2 (tGFP-tagged) - Mouse tetraspanin 2 (Tspan2)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tspan2
Synonyms 6330415F13Rik; B230119D02Rik; tspan-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202532 representing NM_027533
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCGTTTTCGCGGGGGCCTGCGGTGCATCAAGTATCTGCTGCTCGGCTTCAACCTGCTGTTCTGGC
TAGCCGGATCAGCCGTTATTGCATTTGGACTATGGTTTCGATTTGGAGGTACCATGAAGGATTTATCATC
AGAGGACAAGTCCCCAGAGTATTTCTATGTGGGGCTGTATGTGCTGGTTGGTGCTGGGGCCCTGATGATG
ACTGTGGGTTTCTTCGGGTGCTGTGGAGCCATGCGTGAGTCACAGTGTGTGCTCGGATCATTTTTTACCT
GTCTGTTGGTGATATTTGCTGCTGAAGTAACCACTGGAGTATTTGCATTTATAGGCAAGGATGTAGCTAT
ACGACATGTACAGAGCATGTATGAGGAGGCCTACAGCGACTACCTTAAAGACAGGGCAAGAGGAAATGGA
ACTCTCATTACCTTCCACTCAGCATTTCAGTGCTGTGGAAAAGAAAGCTCTGAGCAAGTCCAGCCCACGT
GTCCAAAGGAGCTCCCAGGCCACAAGAATTGCATCGACAAAATTGAAACCGTAATCAGTGCTAAACTGCA
GCTCATTGGAATTGTTGGTATTGGGATTGCGGGTCTCACGATCTTTGGCATGATATTCAGCATGGTGCTT
TGCTGTGCAATACGGAACTCACGTGATGTGATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202532 representing NM_027533
Red=Cloning site Green=Tags(s)

MGRFRGGLRCIKYLLLGFNLLFWLAGSAVIAFGLWFRFGGTMKDLSSEDKSPEYFYVGLYVLVGAGALMM
TVGFFGCCGAMRESQCVLGSFFTCLLVIFAAEVTTGVFAFIGKDVAIRHVQSMYEEAYSDYLKDRARGNG
TLITFHSAFQCCGKESSEQVQPTCPKELPGHKNCIDKIETVISAKLQLIGIVGIGIAGLTIFGMIFSMVL
CCAIRNSRDVI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027533
ORF Size 663 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027533.2, NP_081809.2
RefSeq Size 4243 bp
RefSeq ORF 666 bp
Locus ID 70747
UniProt ID Q922J6
Cytogenetics 3 F2.2
Summary May play a role in signalling in oligodendrocytes in the early stages of their terminal differentiation into myelin-forming glia and may also function in stabilizing the mature sheath.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027533" in other vectors (4)
SKU Description Size Price
MC200518 Tspan2 (untagged) - Mouse tetraspanin 2 (Tspan2), (10ug) 10 ug
$300.00
MR202532 Tspan2 (Myc-DDK-tagged) - Mouse tetraspanin 2 (Tspan2) 10 ug
$289.00 $300.00
MR202532L3 Lenti ORF clone of Tspan2 (Myc-DDK-tagged) - Mouse tetraspanin 2 (Tspan2) 10 ug
$600.00
MR202532L4 Lenti ORF clone of Tspan2 (mGFP-tagged) - Mouse tetraspanin 2 (Tspan2) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.