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Nt5m (NM_134029) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202498
Nt5m (tGFP-tagged) - Mouse 5',3'-nucleotidase, mitochondrial (Nt5m)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nt5m
Synonyms 2010013E09Rik; AI846937; dNT-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202498 representing NM_134029
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATAGGCTGCGCGGCTGCTGTGCGCGTCCCCGCGGTGCCCCGCTACGGGCCGAGCGGAGCCGGGCGA
GCAGCCGCGCCCTGCGGGTGCTGGTGGACATGGACGGCGTGCTGGCGGACTTTGAGGGCGGCTTCCTCCG
GAAGTTCCGCGCGCGTTTCCCCGACCTGCCCTTCGTGGCGCTGGAGGACCGGCGCGGCTTCTGGGTGTCG
GAGCAGTACGGACGTCTACAGCCTGGGCTAAGCGAGAAGGCGATCAGCATCTGGGAGTCAAAGGATTTCT
TCTTTGAACTTGAGCCTCTCCCTGGAGCCGTGGAAGCTGTGAAGCAGATGGCCAATCTACAGAACACTGA
TGTCTTCATCTGTACAAGCCCCATCAAGATGTTCAAGTACTGTCCCTATGAGAAGTATGCCTGGGTGGAG
AAGCACTTTGGCCCTGACTTTCTGGAGCAGATTGTGTTGACCAGAGACAAGACTGTGATCTCAGCCGACC
TTCTCATAGATGACCGGCCAGACATCACAGGGGCTGAGCCACATCCCAGCTGGGAGCACATCCTCTTTAC
CTCCTGCCACAACTACCACCTGCAGCTGCAGCCCCCTCGCCGCAGGCTGCACTCATGGGCAGACGACTGG
AAGGCCATCTTGGACAGCAAGCGGCTCCGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202498 representing NM_134029
Red=Cloning site Green=Tags(s)

MHRLRGCCARPRGAPLRAERSRASSRALRVLVDMDGVLADFEGGFLRKFRARFPDLPFVALEDRRGFWVS
EQYGRLQPGLSEKAISIWESKDFFFELEPLPGAVEAVKQMANLQNTDVFICTSPIKMFKYCPYEKYAWVE
KHFGPDFLEQIVLTRDKTVISADLLIDDRPDITGAEPHPSWEHILFTSCHNYHLQLQPPRRRLHSWADDW
KAILDSKRLR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_134029
ORF Size 660 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_134029.1
RefSeq Size 1306 bp
RefSeq ORF 663 bp
Locus ID 103850
UniProt ID Q8VCE6
Cytogenetics 11 B1.3
Summary Dephosphorylates specifically the 5' and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides, and so protects mitochondrial DNA replication from excess dTTP. Has only marginal activity towards dIMP and dGMP.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_134029" in other vectors (4)
SKU Description Size Price
MC205764 Nt5m (untagged) - Mouse 5',3'-nucleotidase, mitochondrial (Nt5m), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$300.00
MR202498 Nt5m (Myc-DDK-tagged) - Mouse 5',3'-nucleotidase, mitochondrial (Nt5m), nuclear gene encoding mitochondrial protein 10 ug
$289.00 $300.00
MR202498L3 Lenti ORF clone of Nt5m (Myc-DDK-tagged) - Mouse 5',3'-nucleotidase, mitochondrial (Nt5m), nuclear gene encoding mitochondrial protein 10 ug
$600.00
MR202498L4 Lenti ORF clone of Nt5m (mGFP-tagged) - Mouse 5',3'-nucleotidase, mitochondrial (Nt5m), nuclear gene encoding mitochondrial protein 10 ug
$600.00

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