Thap3 (NM_175152) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202471
Thap3 (tGFP-tagged) - Mouse THAP domain containing, apoptosis associated protein 3 (Thap3)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Thap3
Synonyms 2010013E08Rik; 2210418H06Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202471 representing NM_175152
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGAAGTCTTGCGCGGCCCGGCAATGCTGCAACCGCTACAGCAGCCGCAGGAAGCAGCTCACCTTCC
ACCGGTTCCCCTTCAGCCGCCCGGAGCTGTTGAGGGAGTGGGTGCTCAACATCGGCCGGGCTGACTTCAA
GCCTAAGCAGCACACAGTCATCTGCTCGGAACACTTCAGACCCGAGTGCTTCAGCGCCTTTGGGAACCGC
AAGAACCTGAAACACAATGCTGTGCCCACGGTGTTCGCTTTTCAGAACCCCACAGAGGTCTGCCCTGAGG
TGGGGGCTGGTGGGGACAGCTCAGGGAGGAACATGGACACCACACTGGAAGAACTTCAGCCTCCAACCCC
GGAAGGCCCCGTGCAGCAGGTCTTACCAGATCGAGAAGCAATGGAGGCCACGGAGGCCGCTGGCCTGCCT
GCCAGCCCTCTGGGGTTGAAGAGGCCCCTTCCGGGACAGCCGTCTGATCACAGCTATGCCCTTTCGGACT
TGGATACCCTCAAAAAAAAACTCTTTCTCACACTGAAGGAAAACAAGAGGCTTCGGAAGCGGCTGAAAGC
CCAGAGGCTGCTGTTGCGGAGGACATGTGGCCGCCTGAGAGCCTACAGAGAGGGACAGCCGGGACCTCGG
GCCAGACGGCCGGCACAGGGAAGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202471 representing NM_175152
Red=Cloning site Green=Tags(s)

MPKSCAARQCCNRYSSRRKQLTFHRFPFSRPELLREWVLNIGRADFKPKQHTVICSEHFRPECFSAFGNR
KNLKHNAVPTVFAFQNPTEVCPEVGAGGDSSGRNMDTTLEELQPPTPEGPVQQVLPDREAMEATEAAGLP
ASPLGLKRPLPGQPSDHSYALSDLDTLKKKLFLTLKENKRLRKRLKAQRLLLRRTCGRLRAYREGQPGPR
ARRPAQGS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175152
ORF Size 654 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175152.4, NP_780361.2
RefSeq Size 1128 bp
RefSeq ORF 657 bp
Locus ID 69876
UniProt ID Q8BJ25
Cytogenetics 4 E2
Summary Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175152" in other vectors (4)
SKU Description Size Price
MC207689 Thap3 (untagged) - Mouse THAP domain containing, apoptosis associated protein 3 (Thap3), transcript variant 1, (10ug) 10 ug
$330.00
MR202471 Thap3 (Myc-DDK-tagged) - Mouse THAP domain containing, apoptosis associated protein 3 (Thap3), transcript variant 1 10 ug
$289.00 $300.00
MR202471L3 Lenti ORF clone of Thap3 (Myc-DDK-tagged) - Mouse THAP domain containing, apoptosis associated protein 3 (Thap3), transcript variant 1 10 ug
$600.00
MR202471L4 Lenti ORF clone of Thap3 (mGFP-tagged) - Mouse THAP domain containing, apoptosis associated protein 3 (Thap3), transcript variant 1 10 ug
$600.00

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