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Eif3k (NM_028659) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202466
Eif3k (tGFP-tagged) - Mouse eukaryotic translation initiation factor 3, subunit 12 (Eif3s12)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Eif3k or NM_028659"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Eif3k
Synonyms 1200009C21Rik; Eif3s12
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202466 representing NM_028659
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGATGTTTGAGCAGATGAGAGCGAACGTGGGCAAGTTGCTCAAGGGTATCGACAGGTACAATCCTG
AGAACTTGGCTACCCTGGAGCGATATGTGGAGACACAGGCCAAAGAGAATGCCTATGATCTGGAGGCCAA
CCTGGCCGTCCTGAAACTGTACCAGTTCAACCCAGCCTTCTTCCAGACCACAGTCACTGCCCAGATTCTG
CTGAAAGCCCTCACCAACCTGCCCCACACCGACTTCACTCTGTGTAAATGTATGATCGACCAGGCACATC
AAGAAGAGCGGCCCATCCGGCAGATCTTGTACCTCGGGGACCTGCTGGAGACCTGCCACTTTCAAGCCTT
CTGGCAAGCCCTGGATGAGAACATGGACCTTCTGGAAGGCATAACTGGCTTTGAAGACTCTGTCCGAAAA
TTTATCTGCCACGTGGTGGGCATCACGTACCAGCACATCGACCGCTGGCTGCTGGCCGAGATGCTCGGAG
ACCTGACTGACAACCAGCTGAAGGTGTGGATGAGCAAGTACGGCTGGAGCGCTGACGAGTCAGGGCAGGT
CTTCATCTGCAGCCAGGAAGAGAGCATTAAGCCCAAGAACATCGTGGAGAAGATTGACTTTGACAGTGTG
TCCAGCATCATGGCCTCCTCCCAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202466 representing NM_028659
Red=Cloning site Green=Tags(s)

MAMFEQMRANVGKLLKGIDRYNPENLATLERYVETQAKENAYDLEANLAVLKLYQFNPAFFQTTVTAQIL
LKALTNLPHTDFTLCKCMIDQAHQEERPIRQILYLGDLLETCHFQAFWQALDENMDLLEGITGFEDSVRK
FICHVVGITYQHIDRWLLAEMLGDLTDNQLKVWMSKYGWSADESGQVFICSQEESIKPKNIVEKIDFDSV
SSIMASSQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028659
ORF Size 654 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028659.1, NP_082935.1
RefSeq Size 774 bp
RefSeq ORF 657 bp
Locus ID 73830
UniProt ID Q9DBZ5
Cytogenetics 7 B1
Summary Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028659" in other vectors (4)
SKU Description Size Price
MC207733 Eif3k (untagged) - Mouse eukaryotic translation initiation factor 3, subunit K (Eif3k), (10ug) 10 ug
$330.00
MR202466 Eif3k (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 3, subunit K (Eif3k) 10 ug
$289.00 $300.00
MR202466L3 Lenti ORF clone of Eif3k (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 3, subunit K (Eif3k) 10 ug
$600.00
MR202466L4 Lenti ORF clone of Eif3k (mGFP-tagged) - Mouse eukaryotic translation initiation factor 3, subunit K (Eif3k) 10 ug
$600.00

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