Hprt (NM_013556) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG202453
Hprt (tGFP-tagged) - Mouse hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1)
$650.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Hprt |
| Synonyms | C81579; HPGRT; Hpr; Hprt1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_013556 |
| ORF Size | 654 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_013556.2, NP_038584.2 |
| RefSeq Size | 1349 bp |
| RefSeq ORF | 657 bp |
| Locus ID | 15452 |
| UniProt ID | P00493 |
| Cytogenetics | X 29.31 cM |
| Summary | The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_013556" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC200159 | Hprt (untagged) - Mouse hypoxanthine guanine phosphoribosyl transferase (Hprt), (10ug) | 10 ug |
$450.00
|
|
| MR202453 | Hprt (Myc-DDK-tagged) - Mouse hypoxanthine guanine phosphoribosyl transferase (Hprt) | 10 ug |
$450.00
|
|
| MR202453L3 | Lenti ORF clone of Hprt (Myc-DDK-tagged) - Mouse hypoxanthine guanine phosphoribosyl transferase (Hprt) | 10 ug |
$750.00
|
|
| MR202453L4 | Lenti ORF clone of Hprt (mGFP-tagged) - Mouse hypoxanthine guanine phosphoribosyl transferase (Hprt) | 10 ug |
$750.00
|
|
Citations
Genome-wide genetic screening with chemically mutagenized haploid embryonic stem cells
Forment, Herzog, Coates et al
Nat. Chem. Biol. (2017) 13 (1), 12-14 DOI: 10.1038/nchembio.2226
Forment, Herzog, Coates et al
Nat. Chem. Biol. (2017) 13 (1), 12-14 DOI: 10.1038/nchembio.2226
AI Summary: OriGene’s pCMV6-AC-Hprt-GFP plasmid (MG202453) was used to transfect Hprt mutant clones, enabling functional studies of Hprt in the genome-wide genetic screening described in the paper.
Snippet: Cells from SC_6TG5758069 and SC_6TG5758117 Hprt mutant clones ( Supplementary Data Set 2 ) were transfected with pEGFP-C1 (Clontech) or pCMV6-AC-Hprt-GFP (OriGene, MG202453) using TransIT-LT1 transfec - tion reagent (Mirus) and following manufacturer’s instructions
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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