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Prelid1 (NM_025596) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202413
Prelid1 (tGFP-tagged) - Mouse PRELI domain containing 1 (Prelid1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Prelid1 or NM_025596"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Prelid1
Synonyms 2610524G07Rik; Preli
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202413 representing NM_025596
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGAAGTATTTCCTGGGCCAGAGCGTGCTCCGAAGTTCCTGGGACCAAGTGTTCGCCGCCTTCTGGC
AACGGTACCCGAATCCCTATAGCAAACATGTCTTAACCGAAGACATAGTGCACCGGGAGGTGACCCCCGA
CCAGAAGCTACTGTCCCGGAGACTCCTGACCAAGACCAACAGGATGCCACGTTGGGCAGAGCGACTGTTT
CCTGCCAACGTTGCTCACTCCGTGTACATCCTGGAGGACTCTATTGTGGACCCCCAGAATCAGACCATGA
CCACCTTCACCTGGAACATCAACCATGCCCGGCTGATGGTGGTGGAGGAACGATGTGTTTACTGTGTGAA
CTCTGACAATAGCGGCTGGACCGAAATCCGTCGGGAAGCCTGGGTCTCCTCTAGCTTATTTGGCGTCTCC
AGAGCTGTCCAGGAATTTGGTCTTGCCCGGTTCAAAAGCAATGTGACCAAGACAATGAAGGGCTTTGAAT
ACATCCTGGCCAAGCTACAAGGTGAGGCCCCTTCCAAAACCCTTGTTGAGACAGCCAAAGAGGCCAAAGA
GAAAGCAAAGGAGACCGCACTGGCAGCTACAGAGAAGGCCAAGGACCTGGCCAACAAGGCCGCCACCAAG
CAGCAGCAGCGGCAGCTCGTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202413 representing NM_025596
Red=Cloning site Green=Tags(s)

MVKYFLGQSVLRSSWDQVFAAFWQRYPNPYSKHVLTEDIVHREVTPDQKLLSRRLLTKTNRMPRWAERLF
PANVAHSVYILEDSIVDPQNQTMTTFTWNINHARLMVVEERCVYCVNSDNSGWTEIRREAWVSSSLFGVS
RAVQEFGLARFKSNVTKTMKGFEYILAKLQGEAPSKTLVETAKEAKEKAKETALAATEKAKDLANKAATK
QQQRQLV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025596
ORF Size 651 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025596.5, NP_079872.4
RefSeq Size 1103 bp
RefSeq ORF 654 bp
Locus ID 66494
UniProt ID Q8R107
Cytogenetics 13 B1
Summary Involved in the modulation of the mitochondrial apoptotic pathway by ensuring the accumulation of cardiolipin (CL) in mitochondrial membranes. In vitro, the TRIAP1:PRELID1 complex mediates the transfer of phosphatidic acid (PA) between liposomes and probably functions as a PA transporter across the mitochondrion intermembrane space to provide PA for CL synthesis in the inner membrane. Regulates the mitochondrial apoptotic pathway in primary Th cells. Regulates Th cell differentiation by down-regulating STAT6 thereby reducing IL-4-induced Th2 cell number. May be important for the development of vital and immunocompetent organs (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025596" in other vectors (4)
SKU Description Size Price
MC201658 Prelid1 (untagged) - Mouse PRELI domain containing 1 (Prelid1), (10ug) 10 ug
$300.00
MR202413 Prelid1 (Myc-DDK-tagged) - Mouse PRELI domain containing 1 (Prelid1) 10 ug
$289.00 $300.00
MR202413L3 Lenti ORF clone of Prelid1 (Myc-DDK-tagged) - Mouse PRELI domain containing 1 (Prelid1) 10 ug
$600.00
MR202413L4 Lenti ORF clone of Prelid1 (mGFP-tagged) - Mouse PRELI domain containing 1 (Prelid1) 10 ug
$600.00

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