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Alg14 (NM_024178) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202409
Alg14 (tGFP-tagged) - Mouse asparagine-linked glycosylation 14 homolog (yeast) (Alg14)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Alg14 or NM_024178"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Alg14
Synonyms 5430428G01Rik; AI854024
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202409 representing NM_024178
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAGCATTCTGATTCTAGCTGCTACAGCCGCAGGGCTAGTGATCTTGCTATTCCAGCGACTATGGA
CCGTGCTTGGGCCCCATCACGTCACTCCCCGAGAGTCTCTCAGACTCTTGATCGTGGCTGGATCCGGTGG
ACACACCACTGAGATCTTGAGGCTGGTTGGAAGCTTGTCCAATGCCTATTCACCAAGGCATTATGTCATT
GCTGAGTCTGATGAAATGAGTGCCAAGAAAATCCATTCTCTTGAAGAACTCTCTCGAGCCCAGAATGACT
CTACTACTGAATACCCCAAGTACCACCTTCACCGAATTCCGAGAAGCCGGGAGGTTCGGCAGTCCTGGCT
CTCCTCTGTGTTCACTACCTTCTACTCCATGTGGTTCTCCTTCCCACTGGTTCTCCGAATAAAGCCAGAT
TTGGTGCTGTGTAATGGACCAGGAACATGCGTCCCTATCTGTGTGTCCGCCCTGCTTCTTGGGATACTTG
GAGTGAAGAAAGTGATCATCGTCTATGTCGAGAGCATCTGCCGAGTGGAGACGCTATCCCTGTCAGGGAA
GATCCTGAGGCACCTCTCCGACTACTTCATTGTTCAGTGGCCCACTCTCAAGGAGAAGTACCCCAAGTCT
GTGTACCTGGGGCGAATTGTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202409 representing NM_024178
Red=Cloning site Green=Tags(s)

MLSILILAATAAGLVILLFQRLWTVLGPHHVTPRESLRLLIVAGSGGHTTEILRLVGSLSNAYSPRHYVI
AESDEMSAKKIHSLEELSRAQNDSTTEYPKYHLHRIPRSREVRQSWLSSVFTTFYSMWFSFPLVLRIKPD
LVLCNGPGTCVPICVSALLLGILGVKKVIIVYVESICRVETLSLSGKILRHLSDYFIVQWPTLKEKYPKS
VYLGRIV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024178
ORF Size 651 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024178.1
RefSeq Size 910 bp
RefSeq ORF 654 bp
Locus ID 66789
UniProt ID Q9D081
Cytogenetics 3 G1
Summary Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. Anchors the catalytic subunit ALG13 to the ER (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_024178" in other vectors (4)
SKU Description Size Price
MC207594 Alg14 (untagged) - Mouse asparagine-linked glycosylation 14 homolog (yeast) (Alg14), (10ug) 10 ug
$330.00
MR202409 Alg14 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 14 homolog (yeast) (Alg14) 10 ug
$289.00 $300.00
MR202409L3 Lenti ORF clone of Alg14 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 14 homolog (yeast) (Alg14) 10 ug
$600.00
MR202409L4 Lenti ORF clone of Alg14 (mGFP-tagged) - Mouse asparagine-linked glycosylation 14 homolog (yeast) (Alg14) 10 ug
$600.00

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