Mrpl24 (BC004736) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202380
Mrpl24 (tGFP-tagged) - Mouse mitochondrial ribosomal protein L24 (cDNA clone MGC:7749 IMAGE:3498625)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mrpl24
Synonyms MGC7749, MGC25749, MGC38056
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202380 representing BC004736
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCTCTCAGCCCTGCTGGCCTTGGCATCCAAAGCCACTTCCTCCCCCTTCTACCGCTATGGAATGA
GCCGTCCAGGTTCCATAGCAGACAAAAGGAAGAATCCTCCATGGAGCAGGCGGCGCCCAGTAGTGGTAGA
GCCCATCTCTGATGAAGACTGGCACCTATTCTGTGGAGACATGGTAGAAATCTTAGAAGGCAAGGATGCT
GGGAAGCAAGGCAAAGTAGTCCAAGTCGTTCGGCAGCGAAACTGGGTTGTCCTGGAGGGGTTGAACACGC
ATTACCGATACATTGGCAGAACCAAGGACCACCGAGGGACCATGATCGCTAGTGAAGCCCCCTTGCTTCA
TCACCAGGTCAAGCTAGTGGATCCTGTGGACAGGAAACCCACTGAGATCCAGTGGAGATTTACTGAGGCA
GGAGAACGTGTTCGAGTCTCTACAAGATCTGGGAGAATTATCCCCAAACCTGAATTTCCTAGAGCAGATG
GCATTGTCCCTGAAACATGGACTGATGGCCCCAAGGACACATCAGTGGAAGATGCTCTTGAAAGAACTTA
TGTGCCCCGACTAAAGACATTAGAAGAAGATGTGATGGAGGCCATGGGGATCCAGGAGACTCGAAGATTC
AAGAAAGTTTATTGGTAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202380 representing BC004736
Red=Cloning site Green=Tags(s)

MRLSALLALASKATSSPFYRYGMSRPGSIADKRKNPPWSRRRPVVVEPISDEDWHLFCGDMVEILEGKDA
GKQGKVVQVVRQRNWVVLEGLNTHYRYIGRTKDHRGTMIASEAPLLHHQVKLVDPVDRKPTEIQWRFTEA
GERVRVSTRSGRIIPKPEFPRADGIVPETWTDGPKDTSVEDALERTYVPRLKTLEEDVMEAMGIQETRRF
KKVYWY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC004736
ORF Size 648 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC004736, AAH04736
RefSeq Size 1109 bp
RefSeq ORF 650 bp
Locus ID 67707
Cytogenetics 3 F1
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Documents
Resources
"BC004736" in other vectors (3)
SKU Description Size Price
MR202380 Mrpl24 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L24 (cDNA clone MGC:7749 IMAGE:3498625) 10 ug
$289.00 $300.00
MR202380L3 Lenti ORF clone of Mrpl24 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L24 (cDNA clone MGC:7749 IMAGE:3498625) 10 ug
$600.00
MR202380L4 Lenti ORF clone of Mrpl24 (mGFP-tagged) - Mouse mitochondrial ribosomal protein L24 (cDNA clone MGC:7749 IMAGE:3498625) 10 ug
$600.00

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