Hmgb1 (BC110667) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG202356
Hmgb1 (tGFP-tagged) - Mouse high mobility group box 1 (cDNA clone MGC:117897 IMAGE:5036554)
$650.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Hmgb1 |
| Synonyms | HMG-1; Hmg1; p30; SBP-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | BC110667 |
| ORF Size | 645 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC110667, AAI10668 |
| RefSeq Size | 1731 bp |
| RefSeq ORF | 647 bp |
| Locus ID | 15289 |
| Cytogenetics | 5 89.18 cM |
| Summary | This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"BC110667" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC206835 | Hmgb1 (untagged) - Mouse high mobility group box 1 (cDNA clone MGC:117897 IMAGE:5036554), (10ug) | 10 ug |
$450.00
|
|
| MR202356 | Hmgb1 (Myc-DDK-tagged) - Mouse high mobility group box 1 (cDNA clone MGC:117897 IMAGE:5036554) | 10 ug |
$450.00
|
|
| MR202356L3 | Lenti ORF clone of Hmgb1 (Myc-DDK-tagged) - Mouse high mobility group box 1 (cDNA clone MGC:117897 IMAGE:5036554) | 10 ug |
$750.00
|
|
| MR202356L4 | Lenti ORF clone of Hmgb1 (mGFP-tagged) - Mouse high mobility group box 1 (cDNA clone MGC:117897 IMAGE:5036554) | 10 ug |
$750.00
|
|
Citations
Carbon monoxide ameliorates hepatic ischemia/reperfusion injury via sirtuin1-mediated deacetylation of HMGB1 in rats
Sun, Guo, Yang et al
Liver Transpl. (2017)
Sun, Guo, Yang et al
Liver Transpl. (2017)
AI Summary: In this study, the HMGB1-GFP plasmid from OriGene was used to visualize and track HMGB1 translocation in AML12 cells, helping to demonstrate how carbon monoxide modulates HMGB1 release during hepatic ischemia/reperfusion injury.
Snippet: In vitro, AML12 cells (American Type Culture Collection, Manassas, VA) transfected with HMGB1-GFP plasmid (OriGene, Rockville, MD) for 24 hours were pretreated with either CORM-2 (50 µM) or iCORM-2 (50 µM) for 2 hours and then subjected to ... All 2 versions Cite SaveSaving
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
