Crybb3 (NM_021352) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202296
Crybb3 (tGFP-tagged) - Mouse crystallin, beta B3 (Crybb3)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Crybb3
Synonyms AI852419
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202296 representing NM_021352
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGCAGCACGGAGCACCTGAGCAGGCTGCAGCTGGCAAGAGCCATGGAGGCCTTGGGGGCAGCT
ACAAGGTGACGGTGTACGAGTTAGAGAACTTCCAGGGCAAGCGATGCGAGCTCTCGGCGGAGTGTCCCAA
CCTCACCGACAGCCTGTTGGAGAAGGTGGGCTCCATCCAAGTGGAGTCTGGACCGTGGCTGGCGTTCGAG
CGCAGGGCCTTTCGTGGGGAGCAGTTTGTTCTGGAGAAGGGGGACTACCCTCGCTGGGATGCCTGGTCCA
GCAGCCGACGTAGTGACATTCTCCTGTCCCTCCGTCCTTTGCACATTGATGGCCCAGACCACAAGCTGCA
CCTGTTTGAGAACCCAGCCTTCAGTGGGCGCAAGATGGAGATCGTAGATGACGATGTGCCCAGCCTGTGG
GCCCATGGTTTCCAGGACCGTGTGGCCAGCATCCGTGTCATCAACGGGACGTGGGTGGGCTACGAGTTCC
CGGGCTACCGTGGGCGCCAGTACGTGTTCGAGCGGGGCGAATTCCGCCACTGGAACGAGTGGGACGCCAA
CCAGCCGCAGCTGCAGTCGGTGCGCCGCATCCGCGACCAGAAGTGGCACAAGCGCGGCTGCTTCCTGAGC
AGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202296 representing NM_021352
Red=Cloning site Green=Tags(s)

MAEQHGAPEQAAAGKSHGGLGGSYKVTVYELENFQGKRCELSAECPNLTDSLLEKVGSIQVESGPWLAFE
RRAFRGEQFVLEKGDYPRWDAWSSSRRSDILLSLRPLHIDGPDHKLHLFENPAFSGRKMEIVDDDVPSLW
AHGFQDRVASIRVINGTWVGYEFPGYRGRQYVFERGEFRHWNEWDANQPQLQSVRRIRDQKWHKRGCFLS
S

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021352
ORF Size 633 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021352.3
RefSeq Size 948 bp
RefSeq ORF 636 bp
Locus ID 12962
UniProt ID Q9JJU9
Cytogenetics 5 55.38 cM
Summary Crystallins are the dominant structural components of the vertebrate eye lens.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_021352" in other vectors (4)
SKU Description Size Price
MC205393 Crybb3 (untagged) - Mouse crystallin, beta B3 (Crybb3), transcript variant 1, (10ug) 10 ug
$300.00
MR202296 Crybb3 (Myc-DDK-tagged) - Mouse crystallin, beta B3 (Crybb3), transcript variant 1 10 ug
$289.00 $300.00
MR202296L3 Lenti ORF clone of Crybb3 (Myc-DDK-tagged) - Mouse crystallin, beta B3 (Crybb3), transcript variant 1 10 ug
$600.00
MR202296L4 Lenti ORF clone of Crybb3 (mGFP-tagged) - Mouse crystallin, beta B3 (Crybb3), transcript variant 1 10 ug
$600.00

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