Ptms (BC080800) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202287
Ptms (tGFP-tagged) - Mouse parathymosin (cDNA clone MGC:91196 IMAGE:4945458)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ptms
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202287 representing BC080800
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGATGATTTGGACTTCGAGACAGGAGATGCAGGGGCCTCAGCCACCTTCCCAATGCAGTGCTCAG
CATTACGTAAGAATGGTTTTGTGGTGCTCAAAGGCCGGCCATGTAAGATCGTCGAGATGTCTACTTCGAA
GACTGGCAAGCATGGCCATGCCAAGGTCCATCTGGTTGGCATTGACATTTTTACTGGGAAGAAATATGAA
GATATCTGCCGCCGCCGCCACCGCGCCAAGTTCCGGCCGCGGCCACCCTCCGCCGTCCAGGGCTCCTCCG
CCTCGGCCCCGGGACCCCGGCTCCCTGCCAGCCCCGGCCCCGGCACCATGTCGGAGAAGAGCGTGGAGGC
AGCGGCCGAGCTGAGTGCCAAGGACCTGAAAGAAAAGAAGGACAAGGTGGAGGAGAAGGCCGGCCGGAAA
GAACGGAAGAAAGAAGTAGTGGAGGAGGAGGAGAATGGAGCTGAGGAAGAGGAAGAAGAAACTGCAGAGG
ATGGAGAGGATGATGATGAAGGGGATGAAGAAGATGAGGAAGAAGAAGAGGAGGAGGATGAAGGCCCCGT
GCGGAAGAGAACTGCTGAAGAGGAGGATGAAGCAGACCCCAAGAGGCAGAAGACAGAAAACGGGGCGTCG
GCA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202287 representing BC080800
Red=Cloning site Green=Tags(s)

MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYE
DICRRRHRAKFRPRPPSAVQGSSASAPGPRLPASPGPGTMSEKSVEAAAELSAKDLKEKKDKVEEKAGRK
ERKKEVVEEEENGAEEEEEETAEDGEDDDEGDEEDEEEEEEEDEGPVRKRTAEEEDEADPKRQKTENGAS
A

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC080800
ORF Size 633 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC080800
RefSeq Size 1265 bp
RefSeq ORF 635 bp
Locus ID 69202
Cytogenetics 6 F2
Summary Parathymosin may mediate immune function by blocking the effect of prothymosin alpha which confers resistance to certain opportunistic infections.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC080800" in other vectors (3)
SKU Description Size Price
MR202287 Ptms (Myc-DDK-tagged) - Mouse parathymosin (cDNA clone MGC:91196 IMAGE:4945458) 10 ug
$289.00 $300.00
MR202287L3 Lenti ORF clone of Ptms (Myc-DDK-tagged) - Mouse parathymosin (cDNA clone MGC:91196 IMAGE:4945458) 10 ug
$600.00
MR202287L4 Lenti ORF clone of Ptms (mGFP-tagged) - Mouse parathymosin (cDNA clone MGC:91196 IMAGE:4945458) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.