Nudt8 (NM_025529) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202277
Nudt8 (tGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 8 (Nudt8)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nudt8
Synonyms 2310039H17Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202277 representing NM_025529
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCCTGATGGCCTGTCCGCGGAGGACGAGCAGCGCTGCCGGCAGCTGCTAGCACGGACCACTGCCC
GGTTACGCTCGCGGCCCGCAGCGGCCGCAGTGCTTGTGCCGCTGTGCCTCGTGCGCGGGGTCCCCGCGTT
GCTCTACACGCTGCGTTCTAGTCGCCTGGTTGGGAGACACAAAGGGGAAGTCAGTTTCCCAGGCGGTAAG
TGTGATCCCGACGACCAAGATGTAATACATACGGCCCTTCGGGAGACTCAGGAGGAGCTGGGCCTAGAGG
TGCCCAAGGAGCACGTGTGGGGTGTCCTGCAGCCAGTGTATGACCGGGAAAAGGCAACCATAGTCCCAGT
ACTTGCCAACGTGGGCCCACTGGATCTGCAGAGCCTTAGGCCCAACCTTGAGGAGGTGGATGAAGTATTT
GAGATGTCTCTGGCCCACTTGCTGCAGACACAGAACCAGGGGTATACCCACTTCTGCCAGGGTGGCCACT
TCAGCTACACACTGCCTGTCTTCTTGCATGGACCACACCGTGTCTGGGGCCTCACAGCTGTCATCACTGA
GCTCACCCTGAAGCTGCTGGCCCCTGGCTTCTACCAGCCCTCCCTAGCTGTTCCTGAGCTGCCTAGGGGT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202277 representing NM_025529
Red=Cloning site Green=Tags(s)

MLPDGLSAEDEQRCRQLLARTTARLRSRPAAAAVLVPLCLVRGVPALLYTLRSSRLVGRHKGEVSFPGGK
CDPDDQDVIHTALRETQEELGLEVPKEHVWGVLQPVYDREKATIVPVLANVGPLDLQSLRPNLEEVDEVF
EMSLAHLLQTQNQGYTHFCQGGHFSYTLPVFLHGPHRVWGLTAVITELTLKLLAPGFYQPSLAVPELPRG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025529
ORF Size 630 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025529.3, NP_079805.1
RefSeq Size 787 bp
RefSeq ORF 633 bp
Locus ID 66387
UniProt ID Q9CR24
Cytogenetics 19 A
Summary Probably mediates the hydrolysis of some nucleoside diphosphate derivatives.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025529" in other vectors (4)
SKU Description Size Price
MC207578 Nudt8 (untagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 8 (Nudt8), (10ug) 10 ug
$330.00
MR202277 Nudt8 (Myc-DDK-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 8 (Nudt8) 10 ug
$289.00 $300.00
MR202277L3 Lenti ORF clone of Nudt8 (Myc-DDK-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 8 (Nudt8) 10 ug
$600.00
MR202277L4 Lenti ORF clone of Nudt8 (mGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 8 (Nudt8) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.