OriGene Logo

Polr2e (NM_025554) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG202269
Polr2e (tGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide E (Polr2e)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8
100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "Polr2e or NM_025554"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Polr2e
Synonyms 25kDa; 2410021N14Rik; AW208866; RPB5; XAP4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202269 representing NM_025554
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGACGAGGAGGAGACCTACCGGCTGTGGAAGATCCGCAAGACGATCATGCAGCTGTGTCATGACC
GTGGCTACCTGGTGACCCAGGATGAACTGGACCAGACACTTGAGGAATTCAAGGCGCAGTTTGGGGACAA
GCCCAGCGAAGGACGGCCAAGGCGCACAGACCTCACGGTGCTGGTGGCCCACAACGATGACCCCACAGAC
CAGATGTTTGTGTTCTTCCCAGAGGAGCCCAAGGTGGGCATCAAGACCATCAAGGTATACTGCCAGCGCA
TGCAGGAGGAAAACATCACACGGGCGCTGATTGTGGTGCAGCAGGGCATGACGCCCTCCGCCAAGCAGTC
CCTGGTGGACATGGCCCCGAAGTATGTGCTGGAACAGTTTCTACAGCAGGAGCTGCTCATCAATATCACA
GAGCACGAGCTAGTCCCTGAGCACGTGGTCATGACAAAGGAGGAGGTGACTGAACTGCTGGCTCGATACA
AGCTTCGTGAGAGCCAGCTACCCAGAATCCAGGCCGGGGACCCAGTGGCACGGTACTTTGGGATCAAGCG
AGGGCAGGTCGTGAAGATCATCCGCCCCAGTGAGACGGCTGGCCGCTACATCACCTACCGCCTGGTGCAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202269 representing NM_025554
Red=Cloning site Green=Tags(s)

MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVAHNDDPTD
QMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAPKYVLEQFLQQELLINIT
EHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARYFGIKRGQVVKIIRPSETAGRYITYRLVQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025554
ORF Size 630 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025554.2, NP_079830.2
RefSeq Size 1022 bp
RefSeq ORF 633 bp
Locus ID 66420
UniProt ID Q80UW8
Cytogenetics 10 C1
Summary DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_025554" in other vectors (4)
SKU Description Size Price
MC207583 Polr2e (untagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide E (Polr2e), (10ug) 10 ug
$330.00
MR202269 Polr2e (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide E (Polr2e) 10 ug
$289.00 $300.00
MR202269L3 Lenti ORF clone of Polr2e (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide E (Polr2e) 10 ug
$600.00
MR202269L4 Lenti ORF clone of Polr2e (mGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide E (Polr2e) 10 ug
$600.00

Welcome to OriGene AI!

I'm your AI-powered guide to everything OriGene.
Ask me anything:

  • Product specs
  • Ordering help
  • Technical questions
  • How to find exactly what your experiment needs

Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.

Velaro Chat Img

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.