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Fgf10 (NM_008002) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202235
Fgf10 (tGFP-tagged) - Mouse fibroblast growth factor 10 (Fgf10)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
  $650.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fgf10
Synonyms AEY17; BB213776; Fgf-10; Gsfaey17
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202235 representing NM_008002
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGAAATGGATACTGACACATTGTGCCTCAGCCTTTCCCCACCTGCCGGGCTGCTGTTGCTGCTTCT
TGTTGCTCTTTTTGGTGTCTTCGTTCCCTGTCACCTGCCAAGCTCTTGGTCAGGACATGGTGTCACAGGA
GGCCACCAACTGCTCTTCTTCCTCCTCGTCCTTCTCCTCTCCTTCCAGTGCGGGAAGGCATGTGCGGAGC
TACAATCACCTCCAAGGAGATGTCCGCTGGAGAAGGCTGTTCTCCTTCACCAAGTACTTTCTCACGATTG
AGAAGAACGGCAAGGTCAGCGGGACCAAGAATGAAGACTGTCCGTACAGTGTCCTGGAGATAACATCAGT
GGAAATCGGAGTTGTTGCCGTCAAAGCCATCAACAGCAACTATTACTTAGCCATGAACAAGAAGGGGAAA
CTCTATGGCTCAAAAGAGTTTAACAACGACTGTAAGCTGAAAGAGAGAATAGAGGAAAATGGATACAACA
CCTATGCATCTTTTAACTGGCAGCACAATGGCAGGCAAATGTATGTGGCATTGAATGGAAAAGGAGCTCC
CAGGAGAGGACAAAAAACAAGAAGGAAAAACACCTCTGCTCACTTCCTCCCCATGACGATCCAAACA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202235 representing NM_008002
Red=Cloning site Green=Tags(s)

MWKWILTHCASAFPHLPGCCCCFLLLFLVSSFPVTCQALGQDMVSQEATNCSSSSSSFSSPSSAGRHVRS
YNHLQGDVRWRRLFSFTKYFLTIEKNGKVSGTKNEDCPYSVLEITSVEIGVVAVKAINSNYYLAMNKKGK
LYGSKEFNNDCKLKERIEENGYNTYASFNWQHNGRQMYVALNGKGAPRRGQKTRRKNTSAHFLPMTIQT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008002
ORF Size 627 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008002.4
RefSeq Size 3880 bp
RefSeq ORF 630 bp
Locus ID 14165
UniProt ID O35565
Cytogenetics 13 67.14 cM
Summary Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008002" in other vectors (6)
SKU Description Size Price
MC207273 Fgf10 (untagged) - Mouse fibroblast growth factor 10 (Fgf10), (10ug) 10 ug
$450.00
MR202235 Fgf10 (Myc-DDK-tagged) - Mouse fibroblast growth factor 10 (Fgf10) 10 ug
$450.00
MR202235L1 Lenti ORF clone of Fgf10 (Myc-DDK-tagged) - Mouse fibroblast growth factor 10 (Fgf10) 10 ug
$750.00
MR202235L2 Lenti ORF clone of Fgf10 (mGFP-tagged) - Mouse fibroblast growth factor 10 (Fgf10) 10 ug
$750.00
MR202235L3 Lenti ORF clone of Fgf10 (Myc-DDK-tagged) - Mouse fibroblast growth factor 10 (Fgf10) 10 ug
$750.00
MR202235L4 Lenti ORF clone of Fgf10 (mGFP-tagged) - Mouse fibroblast growth factor 10 (Fgf10) 10 ug
$750.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.