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Spryd4 (NM_025716) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202189
Spryd4 (tGFP-tagged) - Mouse SPRY domain containing 4 (Spryd4)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Spryd4
Synonyms 4633402N23Rik; AA114801
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202189 representing NM_025716
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGCCCTTTGCACGCTGTTGGAAGTTGTACCGCTGGGGCACCAAGCGATGGGGGGTCCCTGCTG
TGGAATCCCGGAGAGGCATCAGTTTCAAACTGGAAGAAAAAACGGCCCACAGCAGCCTGGCACTCTTCAG
AGGCGACACGGGTGTCAAATATGGCTTGGTGGGACTGGAACCCACCAAGGTGGCCCTGAATTTGGAGCGC
TTCCGGGAGTGGGCAGTGGTGCTCGGAGACACCACAGTCACCAGTGGCAGACACTACTGGGAGGTAACAG
TGAAGCGCTCCCAGCAGTTCCGGATAGGAGTGGCAGACGTGGACATGTCCCGGGACAGCTGCGTTGGTGC
TGATGATCGTTCCTGGGTGTTCTCCTATGCCCAGCGCAAGTGGCACAGCATGTTGGCCAACGAAAAAGCT
CCAATTAAGGGTATTGGGCAACCAGAGAAGGTGGGGCTGCTGCTGGACTATGAGGCAAAGAAGCTGAGCC
TGGTGGATGTGAGTCGGATCTCTGTGATCCACACACTACAGACAGATTTCCGGGGCCCAGTGGCACCTGC
TTTTGCTCTTTGGGATGGAGAGCTGCTAACCCACTCAGGACTCGAAGTGCCCAAGGGCCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202189 representing NM_025716
Red=Cloning site Green=Tags(s)

MALPFARCWKLYRWGTKRWGVPAVESRRGISFKLEEKTAHSSLALFRGDTGVKYGLVGLEPTKVALNLER
FREWAVVLGDTTVTSGRHYWEVTVKRSQQFRIGVADVDMSRDSCVGADDRSWVFSYAQRKWHSMLANEKA
PIKGIGQPEKVGLLLDYEAKKLSLVDVSRISVIHTLQTDFRGPVAPAFALWDGELLTHSGLEVPKGL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025716
ORF Size 621 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025716.2, NP_079992.1
RefSeq Size 1570 bp
RefSeq ORF 624 bp
Locus ID 66701
UniProt ID Q91WK1
Cytogenetics 10 D3
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Documents
Resources
"NM_025716" in other vectors (4)
SKU Description Size Price
MC205490 Spryd4 (untagged) - Mouse SPRY domain containing 4 (Spryd4), (10ug) 10 ug
$300.00
MR202189 Spryd4 (Myc-DDK-tagged) - Mouse SPRY domain containing 4 (Spryd4) 10 ug
$289.00 $300.00
MR202189L3 Lenti ORF clone of Spryd4 (Myc-DDK-tagged) - Mouse SPRY domain containing 4 (Spryd4) 10 ug
$600.00
MR202189L4 Lenti ORF clone of Spryd4 (mGFP-tagged) - Mouse SPRY domain containing 4 (Spryd4) 10 ug
$600.00

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